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発がんとがん予防における腸内フローラの役割の解明と機能的腸内菌の開発

阐明肠道菌群在致癌和癌症预防中的作用以及功能性肠道细菌的发育

基本信息

批准号：
10151235
负责人：
大西克成
金额：
$ 1.66万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10151235/
关键词：
腸内菌抗生物質 PhIP azoxymethane ACF リコペン機能的腸内菌

项目摘要

1. 抗菌スペクトルの異なる抗生物質 bacitracin-neomycin-streptomycin(BcNmSm)、metronidazole(MNZ)またはkanamycinで処理したラットに、大腸発癌物質2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP)を投与し、大腸aberrant crypt foci(ACF)数、DNA付加体量を調べた。その結果、ACF数はBcNmSmを投与したラットで有意に減少していた。しかし、DNA付加体レベルは各群間で有意な差がなかった。2. 腸管内でPhIPの解毒抱合体を脱抱合する腸内菌叢由来のβ-glucuronidase、cysteine conjugate β-lyaseの活性を、上記の抗生物質処理を行ったラットの糞便を用いて測定した。その結果、β-glucuronidase活性およびcysteine conjugate β-lyase活性は、いずれの抗生物質によっても低下していた。3. 各種腸内菌培養液を経口投与したラットにおけるPhIP誘発大腸ACF形成を観察すると、Bacteroides fragilis ATCC25285株、Clostridium perfringens GAI0668株、Clostridium butyricum NCIB7423株およびEscherichia coli W3110株を投与したラットではACF形成が培養液無投与PhIP群に比べて有意に抑制されていた。4. リコペン産生プラスミド保有大腸菌の菌体をラットに投与し、大腸発癌物質azoxymethaneによって誘発されるACF数が抑制されるかどうかを調べた。その結果、リコペン産生大腸菌投与群ではベクタープラスミド保有大腸菌を投与した場合に比べてACF数が有意に減少していた。さらに、4個以上のaberrant cryptを持つfocusの数も有意に減少していた。

1. Antimicrobial agents, antibiosis bacitracin-neomycin-streptomycin (BcNmSm), metronidazole (MNZ) antibiotics, kanamycin antibiosis, antibiologics, antibiosis, metronidazole (MNZ), antibiosis, antibiologics, antibiosis, antibiologics, metronidazole (MNZ), antibiologics, antibiologics, antibiosis, antibiologics, antibiosis, antibiologics, antibi The result of the experiment, the number of ACF, the number of BcNmSm, and the number of investments are intended to reduce the number of errors. There is an intention to make a difference between different groups of people who are interested in receiving payment and DNA payment. two。 The detoxification of PhIP in tube, the detoxification of clasp, the activity of β-glucuronidase and cysteine conjugate β-lyase, the activity of anti-biotic, the activity of anti-biotic, the activity of β-lyase, the activity of anti-biotic and anti-biotic. The results showed that the activity of β-glucuronidase, the activity of cysteine conjugate β-lyase, the activity of anti-biotics, the activity of β-lyase and the activity of β-lyase were decreased. 3. All kinds of bacteria culture solution were injected orally and tested. PhIP ACF was formed. Escherichia coli W3110 strain, Bacteroides fragilis ATCC25285 strain, Clostridium perfringens GAI0668 strain and Clostridium butyricum NCIB7423 strain were injected into the culture solution to form a culture ACF. Compared with the PhIP group, the culture solution was intentionally used to inhibit the bacteria. 4. In the first place, the bacteria were kept, the bacteria were injected into the bacteria, and the cancer was azoxymethane. The number of ACF was inhibited. According to the results of the experiment, the results showed that the number of bacteria in the population was significantly lower than that in ACF. The number of customers with more than 4 aberrant crypt accounts holding focus is interested in reducing the number of customers.