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Protein-protein interaction in intracellular signal transduction

细胞内信号转导中的蛋白质-蛋白质相互作用

基本信息

批准号：
10179103
负责人：
INAGAKI Fuyuhiko
金额：
$ 211.33万
依托单位：
HOKKAIDO UNIVERSITY (1999-2001)Tokyo Metropolitan Organization for Medical Research (1998)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

The aim of the present research project is to elucidate the intracellular signal transduction based on the three dimensional structure of proteins and protein complexes. For this purpose, we organized three groups ; The first group was concerned with the intracellular signal transduction including regulational mechanism of actin cytoskeleton and growth factor receptor signaling. The second group was to investigate the signal transduction in the nucleus, where signals were integrated by co-activators. The third group focused on the development of new techniques to elucidate the protein-protein interaction and to establish the method for high-throughout structural determination by NMR. Owing to the nature of interdisciplinary research, molecular biologists, cell biologists and structural biologists joined each group. The supervising group was also organized to set up the research directions and to promote collaborations among each group. In the present research project, a number of joint researches were made in four years from 1998 till 2001 and more than 80 novel structures of proteins and ligand-protein complexes were determined and published in distinguished international journals. We invited many young scientists as members of the project. We also organized NMR workshops where we invited Dr. James Keeler of Cambridge University who has reputation for teaching basic NMR concepts and advanced NMR techniques. The research project contributed significantly not only to promote the exchange of knowledge of interdisciplinary areas but also to develop structural biology in Japan.

本研究项目的目的是阐明基于蛋白质和蛋白质复合物的三维结构的细胞内信号转导。为此，我们组织了三个小组；第一组关注细胞内信号转导，包括肌动蛋白细胞骨架和生长因子受体信号传导的调节机制。第二组是研究细胞核中的信号转导，其中信号由共激活剂整合。第三组重点开发新技术来阐明蛋白质-蛋白质相互作用，并建立核磁共振高通量结构测定方法。由于跨学科研究的性质，分子生物学家、细胞生物学家和结构生物学家加入了每个小组。还成立了指导小组，确定研究方向并促进各小组之间的合作。在本研究项目中，从1998年到2001年的四年间，进行了多项联合研究，确定了80多个蛋白质和配体-蛋白质复合物的新结构，并在国际著名期刊上发表。我们邀请了许多年轻科学家作为该项目的成员。我们还组织了 NMR 研讨会，邀请了剑桥大学的 James Keeler 博士，他在教授基本 NMR 概念和高级 NMR 技术方面享有盛誉。该研究项目不仅为促进跨学科领域的知识交流做出了重大贡献，而且还为日本结构生物学的发展做出了重大贡献。