权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

ミヤコグサ根粒形成の分子遺伝学的解析

莲藕根瘤形成的分子遗传学分析

基本信息

批准号：
10182209
负责人：
川口正代司
金额：
$ 1.92万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10182209/
关键词：
ミヤコグサ根粒形成変異体 sym 共生

项目摘要

網羅的根粒形成突然変異体の単離と遺伝解析による宿主遺伝子群の顕在化L.japonicus Gifu B-129の8000種子にEMSで変異を導入し、約4万のM2種子から少なくともM4世代まで形質が安定に遺伝する32系統の共生変異体を単離した。根粒形成の変異体は大きく2つのカテゴリーに区分された。有効根粒は形成されるものの数に異常を示すもの(5系統)と有効根粒に至る発生過程のもの(27系統)であった。以下遺伝解析の結果明らかになった根粒の着生効率と発生過程に異常を示す共生変異体symである。A 有効根粒の数の制御(1) 有効根粒が増加する変異体a) 寒天培地上で黒い根粒を形成する変異体dark-complextioned nodules,dcn(sym77)b) 根粒過剰着生変異体sym78(2) 有効根粒が減少する変異体a) 根毛欠損の変異体slipping root,slpb) tsrに似た根をもつ変異体radial organizational,rorlB 有効根粒に至るまでの発生過程における変異体有効根粒にまで至る発生過程の変異体は大きくNod-,Nor-,Fix-に分離された。27系統の変異体から発生過程を代表する7遺伝子座を同定した。Nor-とは今回ミヤコグサの変異体の表現型を詳細に観察した結果新たにもうけたNodule organogesisにおける変異体のカテゴリーであり、Nod-とFix-の間の発生過程に位置するものである。(1) 根粒非着生変異体Nod- sym70,sym71,sym72(2) 無効根粒変異体Fix- sym75,fen1(sym76)(3) 根粒の器官形成過程に異常をもつ変異体Nor- alb1(sym74-1),sym74-2(4) メンデル遺伝を示さないpleiotropicな変異体sym79

The root grain formation of the network suddenly affects the isolation of the host subgroup of the L.japonicus Gifu B. there are more than 8000 EMS hosts in the network, and about 40,000 M2 in the M4 generation. the system is stable and stable. The root grains are divided into different parts of the root. The number of root cells in the system (5 system) is very high, which indicates that there is a problem in the process (system 27). The following analytical results show that the root growth rate and the growth process often show that symbiotic sym genes are not present. A has the control of the number of root grains (1) the number of root grains is controlled (1) the root grains of black mackerel are cultured on the ground in cold weather to form the body dark-complextioned nodules,dcn (sym77) b) the root grains pass through the growth body sym78 (2) there are few root grains, a) the root hairs are deficient, and the body slipping root,slpb) tsr resembles the body radial organizational of the root hair. In rorlB, there are some problems in the process of life from root to mother, and there are some problems in the process of life. There is a lot of information about how to separate students from root to Nod-,Nor-,Fix-. The process of the 27-year-old students is the same as that of the 7-year-old students. Nor- has completed a series of health tests this time around. The results show that there is a significant increase in the number of Nodule organogesis patients, and that the location of the health process is very important. (1) non-vegetative Nod- sym70,sym71,sym72 (2) Fix- sym75,fen1 (sym76) (3) the process of organ formation in root grains is the formation of normal chromosomes Nor- alb1 (sym74-1), sym74-2 (4) the expression of sym79 is sensitive to the growth of eukaryotic cells.