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In vivo measurement of ocular circulation with the Laser Speakle Method. Development of apparatus and application in ophthalmological research.

使用激光 Speakle 方法体内测量眼循环。

基本信息

批准号：
10357016
负责人：
ARAIE Makoto
金额：
$ 15.94万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10357016/
关键词：
Laser speckle Blood velocity Tissue circulation Clinical application Optic. nerve head Choroid Retinal vessel

项目摘要

We have measured the in vivo blood velocity in human retinal veins using a laser speckle system. We also tried to measure whether the normalized blur (NB) value for calculating the retinal venule blood velocity was applicable to the retinal veins near the disc. The NB value, however, had an upper measurement limit. We have constructed a new apparatus for clinical application and also adopted the square blur rate (SBR), a quantitative index of blood velocity, for measuring retinal blood velocity near the disc This system consists of an infrared CCD camera, a monitor display system, a diode laser, an image sensor, and a high speck personal computer including Windows 98-based analyzing system. The funds area, including a target retinal vein, is illuminated with a diode laser through a fundus camera and the laser speckle pattern is imaged onto the area sensor. From the time change of the contrast of the speckle pattern, laser intensity and deviation are calculated using a logic board and the SBR value is also calculated using a personal computer. In an in vitro experiment the SBR obtained from blood flow in a glass capillary tube, used as an analogue of a retinal vein, was correlated with the diameter of the tube, the actual blood flow rate, and the background SBR value, which was used as an analogue of choroidal circulation. In the in vivo experiment the blood velocity in human retinal veins of approximately 150 μm in diameter was estimated in 16 normal human eyes using monograms based on the results of the in vitro experiment. Velocity averaged 18.4 ± 3.8mm/s (mean ± SD, n = 16) in retinal veins of 146 ± 6μm diameter. The coefficient of reproducibility of 2-minute interval measurements was 3.6 ± 0.9 %, and it took 60 ± 11s for one measurement The present methodology is clinically valid for measuring blood velocity in retinal veins.

我们用激光散斑系统测量了人视网膜静脉的体内血流速度。我们还尝试测量用于计算视网膜小静脉血流速度的归一化模糊（NB）值是否适用于靠近椎间盘的视网膜静脉。然而，NB值有一个测量上限。本文构建了一种临床应用的新型仪器，采用方阵模糊率（SBR）作为血流速度的定量指标来测量视网膜近盘血流速度，该系统由红外CCD摄像机、监控显示系统、二极管激光器、图像传感器和高点个人计算机（包括基于Windows 98的分析系统）组成。眼底区域，包括目标视网膜静脉，通过眼底相机用二极管激光照射，激光散斑图案成像到区域传感器上。根据散斑图对比度的时间变化，利用逻辑板计算激光强度和偏差，并利用个人计算机计算SBR值。在一项体外实验中，通过玻璃毛细管（用作视网膜静脉的模拟物）的血流获得的SBR与管道直径、实际血流速率和背景SBR值（用作脉络膜循环的模拟物）相关。在体内实验中，以体外实验结果为基础，用字母组合图估计了16只正常人视网膜直径约150 μm静脉的血流速度。直径为146±6μm的视网膜静脉平均流速为18.4±3.8mm/s （mean±SD, n = 16）。2分钟间隔测量的再现系数为3.6±0.9%，一次测量耗时60±11s，本方法在临床上是有效的。