Basic and practical investigations on neuro-protective or regenerative treatments of retinal neuropathic ocular

1)分离培养：在培养的大鼠视网膜胶质细胞中，发现缺氧胁迫下未知基因的表达。研究了离心力作用下视网膜神经节细胞和视网膜胶质细胞的分子和细胞反应。2)神经再生：制作眼压升高的神经再生实验大鼠模型眼，定量观察视网膜神经细胞的损伤和再生情况，并对模型的若干特性进行研究。获得真皮干细胞培养后，将其注射到视网膜变性模型大鼠视网膜下。注射后8周发现细胞，观察到神经保护作用。3)大鼠和猴眼的体内实验研究：光凝法在食蟹猴单侧眼小梁网处诱导持续稳定的高眼压。青光眼患者行海德堡视网膜断层扫描（HRT）证实有明显的眼神经头改变。形态学或循环分析使用对侧眼睛作为适当的控制，使这些猴子青光眼模型。全身给予对体循环作用较弱的钙拮抗剂氯美嗪后，正常食蟹猴眼的眼神经头局部循环增加，而我们所研制的青光眼模型眼的这种作用可能减弱。

1)Isolated cultivation :In cultured retinal glial cells of rats, expression of unknown genes was found under the challenge of hypoxia.Molecular and cellular reactions of retinal ganglion cell and retinal glial cell under centrifugal force loading were investigated.2)Neuroregeneration :Experimental rat model eye for surveys on neuroregeneration with increased intraocular pressure in which damages and regeneration of the retinal neural cells were quantitatively observed was produced, and the several properties of the model were investigated.Following dermal stem cells were obtained and cultured, they were injected below the retina of the retinal degeneration-model rats. The cells were found eight weeks after the injection and neuroprotective influence was observed.3)Investigations on rat and monkey eyes in vivo :Persistent and stable higher intraocular pressure was induced by photocoagulation at the trabecular meshwork of unilateral eyes of cynomolgus monkeys. Glaucomatous distinctive ocular nerve head changes were confirmed in those eyes with Heidelberg Retina Tomograph(HRT). Morphological or circulatory analyses using contralateral eyes as appropriate controls are enabled with those monkey glaucoma models.Under systemic administration of lomerizine, a calcium antagonist with weaker effect on the systemic circulation, increased local circulation of ocular nerve head was found in normal cynomolgus monkey eyes and this effect might be decreased in the glaucoma model eyes that we have developed.