Study of fluid dynamics in the vitreous in normal and pathological eges

正常和病理状态下玻璃体的流体动力学研究

基本信息

  • 批准号:
    04671066
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1993
  • 项目状态:
    已结题

项目摘要

It is well known that fluid dynamics in the vitreous depends on the function of retinal pigment epithelium (RPE). We already reported characteristics of mode of fluorescein movement across the isolated rabbit RPE-choroid using Ussing's chamber and that this system is suited for studying the functions of RPE, i.e., active transporting function and barrier function against diffusion of exogenous substances. This time, we studied characteristics of mode of carboxyfluorescein movement across the isolated rabbit RPE-choroid. Carboxyfluorescein is known to be a much better tracer dye in studying the barrier function of cell layrs and it is expected that fluid movement across the RPE can be indirectly guessed by calculating the permeability coefficient of RPE to carboxyfluorescein.Movement of carboxyfluorescein across the isolated RPE-choroid from the choroid to the vitreous side (Inward movement) was found to be by passive diffusion, while its movement from the vitreous to the choroid side (Outward movement) was partly by active transport. At concentration of 30 muM, outward permeability of the isolated rabbit RPE-choroid to carboxyfluorescein was about twice of the inward permeability. Probenecid, ouabain, 2,4-dinitrophenol and hippurate significantly reduced the outward permeability to carboxyfluorescein. A saturation kinetic study revealed that affinity and maximum velocity of reaction of carboxyfluorescein to the carrier is lower than that of fluorescein. The inward permeability to carboxyfluorescein was significantly lower in BSS+GSSG than in BSS or Krebs' Ringer solution, which suggested that GSSG, oxidized glutathione, has beneficial effects on the barrier function of the RPE.The usage of carboxyfluorescein and Ussing's chamber is suggested to be a useful tool for studying active transport function which is associated with fluid movement across the RPE and barrier function of the RPE.
众所周知,玻璃体内的流体动力学取决于视网膜色素上皮(RPE)的功能。我们已经报道了荧光素在分离兔RPE-脉络膜上使用Ussing腔的运动模式的特征,并且该系统适合于研究RPE的功能,即主动运输功能和抵抗外源物质扩散的屏障功能。这一次,我们研究了羧基荧光素在离体兔rpe -脉络膜上运动模式的特征。在研究细胞层的屏障功能方面,羧基荧光素是一种更好的示踪染料,预计通过计算RPE对羧基荧光素的渗透系数可以间接猜测RPE上的流体运动。羧基荧光素通过离体rpe -脉络膜从脉络膜向玻璃体一侧移动(向内移动)是通过被动扩散,而从玻璃体向脉络膜一侧移动(向外移动)部分是通过主动运输。在30mum浓度下,离体兔rpe -脉络膜对羧基荧光素的向外通透性约为向内通透性的两倍。Probenecid、瓦巴因、2,4-二硝基苯酚和hippurate显著降低了对羧基荧光素的外透性。饱和动力学研究表明,羧基荧光素对载体的亲和力和最大反应速度低于荧光素。BSS+GSSG对羧基荧光素的向内通透性显著低于BSS和Krebs’Ringer溶液,说明氧化谷胱甘肽GSSG对RPE的屏障功能有有益作用。羧基荧光素和Ussing室的使用被认为是研究与RPE的流体运动和RPE的屏障功能相关的主动传递功能的有用工具。

项目成果

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ARAIE Makoto其他文献

ARAIE Makoto的其他文献

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{{ truncateString('ARAIE Makoto', 18)}}的其他基金

Damage of retinal ganglion cells : Mechanism of the cell death in the local and central nervous system and protection and regeneration of the neural cells.
视网膜神经节细胞的损伤:局部和中枢神经系统细胞死亡的机制以及神经细胞的保护和再生。
  • 批准号:
    18209053
  • 财政年份:
    2006
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Basic and practical investigations on neuro-protective or regenerative treatments of retinal neuropathic ocular
视网膜神经病性眼病的神经保护或再生治疗的基础和实践研究
  • 批准号:
    14207069
  • 财政年份:
    2002
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Topical medication for the posterior part of the eye. The principles and the investigation of efficient drugs
用于眼睛后部的局部药物。
  • 批准号:
    12557146
  • 财政年份:
    2000
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of readily applicable glaucoma new drugs for lowering intraocular pressure, improvement of ocular circulation, and neuronprotection
开发易于应用的降低眼压、改善眼循环、保护神经元的青光眼新药
  • 批准号:
    11307036
  • 财政年份:
    1999
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
In vivo measurement of ocular circulation with the Laser Speakle Method. Development of apparatus and application in ophthalmological research.
使用激光 Speakle 方法体内测量眼循环。
  • 批准号:
    10357016
  • 财政年份:
    1998
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Analysis of intracellular second messenger systems in ciliary epithelium.
睫状上皮细胞内第二信使系统的分析。
  • 批准号:
    09470377
  • 财政年份:
    1997
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Treatment of glandma. Role of optic nerve head and cheroidal circulation
腺体的治疗。
  • 批准号:
    07457410
  • 财政年份:
    1995
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of an istrument evaluating re tinal circulation in vivo.
开发评估体内视网膜循环的仪器。
  • 批准号:
    06557090
  • 财政年份:
    1994
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
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