VASOMOTOR-ACTIVE FACTORS DERIVED FROM ENDOTHELIALCELLS IN MICE ARTERIES.

有足够的证据表明，生物活性物质从血管内的内皮细胞中释放了生理上和/或病理学上重要的角色，以调节血栓发生或治疗过程的发展。特别是，内皮衍生的约束因子（EDCF）和内皮衍生的放松因子（EDRF）的平衡是非常重要的。我们找到了EDHF（内氟胺衍生的超极化化肥料） ，EDRF中的一种，调节小齿轮直径小于300毫米的活性汽油机音调，大鼠中的直径小于300毫米，and hyperpolarization which EDHF produces in arterial smManagement muscle cells by activating apamin-and charibdotoxin-sensitive K-D1 + JED1 channels decreases Ca-D12 + JED1 sensitivity of contractile elements as well as inhibiting Ca-D12 + D1 influx through L-type Ca D12+ D1 channels。我们还发现了EDHF在维护内功能调节动脉的蒸汽机音调中发挥了一个基本作用，作为一个备份系统来维持EDNO在女性老鼠中的作用。在订购时，将进一步澄清EDHF的生理学或病理学特征及其分子机制，以及更多的有说服力的研究，如那些使用转基因老鼠在未来的未来是必需的。我们发现了一个微型容器灌注系统，它是一个类似实验性方法观察变化的vasomotone of非常小的植物，从小动物这样的老鼠。

There is enough evidence indicating that biologically active substances released from the endothelial cells of blood vessels play physiologically and/or pathophysiologically important roles in regulating thrombogenesis or development of atherosclerosis. Especially, balance of endothelium-derived constricting factors(EDCF) and endothelium-derived relaxing factors(EDRF) is very important. We found that EDHF(endothelium-derived hyperpolarizing faetor) , one of EDRF, regulates actively vasomotor tone of smaller arteries less than 300 mm in diameter in rats, and hyperpolarization which EDHF produces in arterial smooth muscle cells by activating apamin-and charibdotoxin-sensitive KィイD1+ィエD1 channels decreases CaィイD12+ィエD1 sensitivity of contractile elements as well as inhibiting CaィイD12+ィエD1 influx through L-type CaィイD12+ィエD1 channels. We also found that EDHF plays an essential role in maintaining endothelial function regulating vasomotor tone of arteries as a backup system to EDNO in female rats. In order to clarify more the physiological or pathophysiological significance of EDHF and its molecular mechanism, more sophisticated studies such as those using transgenic mice are required in near future. We found that Microvessel Perfusion System is adequate experimental methods observing changes in vasomotor tone of very small arteries obtained from small animals such as mice.