Biomedical responses to changes in body temperature in ciritically ill patients

我们最初测量了血浆中细胞因子和可溶性受体的水平，并研究了它们与全身感染程度和体温的关系。有明显感染时，血浆IL-6、IL-8水平明显升高。我们发现体温与肿瘤坏死因子-α、白介素6和白介素8显著相关。然后，我们分析了意识短暂紊乱且没有任何感染迹象的高热患者，发现血浆中IL-6、IL-8和IL-10的水平最初是高的，然后逐渐下降，这些数据表明高热本身可以诱导细胞因子的产生。在第二个实验中，我们检测了环境温度对体外肝素化全血中IL-8产生的影响。在没有任何刺激的情况下产生少量IL-8。有趣的是，在42℃、40℃孵育时，对内毒素的反应产生IL-8受到相当程度的抑制，但在38℃时不受抑制。结合上述体内研究结果，我们推测高热患者的非血液学细胞可能产生了细胞因子或细胞因子刺激因子。此外，我们还建立了一个用于未来细胞功能分析的系统，如钙反应、细胞表面标志物等。

We initially measured the plasma levels of cytokines, soluble receptors and examined their relationships to the degree of systemic infection and body temperature. In the presence of apparent infection, the plasma IL-6 and IL-8 levels were significantly increased. We found a significant correlation between body temperature and TNF-alpha, IL-6 and IL-8. We then analyzed the hyperthermic patients, whose consciousness was transiently disturbed and who did not show any signs of infection, and found that the plasma levels of IL-6, IL-8 and IL-10 were initially high and then decreased gradually, These data suggested that hyperthermia itself could induce the production of cytokines.In the second experiment, we examined the effect of environmental temperature on IL-8 production in heparinized whole blood in vitro. Small volume of IL-8 was produced without any stimuli. Interestingly, IL-8 production, in response to endotoxin was rather inhibited when incubated at 42℃, 40℃, but not at 38℃. In combination with above in vivo findings, we speculated that cytokine or cytokine-stimulating factor may be produced by no hematological cells in hyperthermic patients. Additionally, we have made a system for future analysis of cell functions, such as calcium response, cell surface markers, etc.