Molecular Basis of Coactivator Function of RNA helicase A.

RNA 解旋酶 A 共激活子功能的分子基础。

• 批准号: 10480196
• 负责人: NAKAJIMA Toshihiro
• 金额: $ 9.92万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10480196/
• 关键词: transcription; helicase; transcriptional coactivator; integrator; splicing; RNA polymerase II; eukaryotic cell; molecular motor; cellular localization

To understand the molecular basis of eukaryotic gene expression system, I have been focusing on the study of transriptional coactivator complexes, especially a signal coactoivator CREB binding protein (CBP) /RNA helicase A/RNA polymerase II complexes. We found the following ((1)-(9)) and believe that the data will contribute the understanding of eukaryotic gene regulation system.(1) Involvement of RNA helicaseA in complex formation of BRCA1 with RNA polymearse II (Nature Genetics 1998).(2) Implication of glycation of c-Fos by Core 2 N-acetylglycosaminyltransferase on enhanced cytokine actions and induced hypertrophic myocardium in transgenic mice (FASEB. J. 1999).(3) Cooperative role of CBP with transcription factor GATA-1 in erythroid differentiation (PNAS. USA 1998).(4) Transcriptional activatity of an autoimmune protein AIRE and its association with common co-activator CBP (J. B. C. in press 2000).(5) Dual roles of RNA helicase A on CREB-dependent transcription (Journal submitted).(6) Molecular mechanism of nuclear import of RNA helicase A (Journal submitted).(7) Association of TAF130 with poly glutamine protein and its implication in apoptosis of neuronal cells (Nature Genetics revised).(8) Interaction of CBP with beta-catenine (Journal submitted).(9) Hypernuclear acetylation in atherosclerotic lesions and activated vascular smooth muscle cells (Biochem. Biophys. Res. Comm. 1999).

为了了解真核生物基因表达系统的分子基础，我一直致力于研究转录辅激活因子复合物，特别是信号辅激活因子CREB结合蛋白（CBP）/RNA解旋酶A/RNA聚合酶II复合物。我们发现以下（1）-（9）），并相信这些数据将有助于了解真核基因调控系统。(1)RNA解旋酶A参与BRCA 1与RNA聚合酶II的复合物形成（Nature Genetics 1998）。(2)核心2 N-乙酰氨基葡萄糖转移酶引起的c-Fos糖基化对转基因小鼠中增强的细胞因子作用和诱导的肥厚心肌的影响（FASEB. J. 1999）。(3)CBP与转录因子加塔-1在红系分化中的协同作用（PNAS.美国1998）。(4)自身免疫蛋白AIRE的转录活性及其与共同辅激活因子CBP的关联（J. B. C. in press 2000）。(5)RNA解旋酶A在CREB依赖性转录中的双重作用（Journal submitted）。(6)RNA解旋酶A的核输入的分子机制（期刊提交）。(7)TAF 130与多聚谷氨酰胺蛋白的关联及其在神经元细胞凋亡中的意义（Nature Genetics修订版）。(8)CBP与β-catenine的相互作用（期刊提交）。(9)动脉粥样硬化病变和活化的血管平滑肌细胞中的超核乙酰化（Biochem.Biophys.2005）。Res. Comm. 1999）。

项目成果

Nakajima T.: "Hypernuclear acetylation in Atherosclerotic lesions and activated vascular smooth muscle cell"Biochem. Biophys. Res. Commun.. 266. 417-427 (1999)

Nakajima T.：“动脉粥样硬化病变中的超核乙酰化和激活的血管平滑肌细胞”Biochem。

Nakajima,T: "Overexpression of Core 2N-acetylglycosaminyltransferase Enhanced Cytokine Actions and Induced Hypertrophic Myocardium in Transgenic Mice"FASEB.J. 13. 2329-2337 (1999)

Nakajima,T：“核心 2N-乙酰糖胺基转移酶的过度表达增强了转基因小鼠的细胞因子作用并诱导肥厚性心肌”FASEB.J。

H. Shin, I. Kitajima, T. Nakajima, Q. Shao, T. Tokioka, I. Takasaki, N. Hanyu, T. Kubo, I. Maruyama.: "Thrombin receptor mediated signals induce expressions of interleukin 6 and granulocyte colony stimulating factor via NF-κB activation in synovial fibrob

H. Shin, I. Kitajima, T. Nakajima, Q. Shao, T. Tokioka, I. Takasaki, N. Hanyu, T. Kubo, I. Maruyama.：“凝血酶受体介导的信号诱导白细胞介素 6 和粒细胞集落的表达通过滑膜纤维中 NF-κB 激活的刺激因子

KP. Sarker, K. Abeyama, JI. Nishi, M. Nakata, T. Tokioka, T. Nakajima, I. Kitajima, I. Maruyama.: "Inhibition of Thrombin-induced Neural Cell Death by Recombinant Thrombomodulin and E5510, a Synthetic Thrombin Receptor Signaling Inhibitor."Thrombosis and

KP。

T.Ohshima: "In vitro and in vivo induction of apoptosis by H-1 parvovirus infection." Journal of General Viology. 79. 3067-3071 (1998)

T.Ohshima：“H-1 细小病毒感染在体外和体内诱导细胞凋亡。”