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Development of a high-energy collision-induced dissociation device for high-sensitivity and high-resolution MS/MS/MS

开发用于高灵敏度和高分辨率MS/MS/MS的高能碰撞诱导解离装置

基本信息

批准号：
10558099
负责人：
TAKAO Toshifumi
金额：
$ 8万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10558099/
关键词：
Magnetic sector mass spectrometer Biomolecule Collision cell Ion transmittance Product ion High-energy collision-induced dissociation Structural analysis 磁場型質量分析

项目摘要

In order to establish high-efficiency MS/MS/MS using a magnetic sector mass spectrometer, a collision cell, which can be potentiated at several kilovolts by high-voltage power supply, has been developed. The cell, which is 1 cm in depth, is embedded into the ion-source block which has been newly designed so as to fit with a commercial magnetic sector mass spectrometer. On the basis of ion transmittance of a singly-charged protonated ion accelerated at a 10-kV potential, the voltages applied to the collision cell and to the acceleration plates (a pair of right and left flat plates), which is situated just behind the cell, have been optimized to be 3.0 kV and 1.5 kV, respectively. This setting allowed almost 80% ion transmittance, based on the intensity of a precursor ion, and could provide the product ions specific for high-energy collision-induced dissociation. It is worth mentioning that the efficiency of ion acquisition and the mass resolution, especially, below the mass. of ca. 20% of a precursor mass, have been much improved. The present device could realize MS/MS/MS from a primary product ion, which has been hardly observed in MS1, in low-mass region, using a magnetic four-sector instrument. It will be efficiently applied for structural analysis of biomolecules.

为了使用扇形磁质谱仪建立高效 MS/MS/MS，开发了一种可以通过高压电源增强至数千伏电压的碰撞池。该池深度为 1 厘米，嵌入新设计的离子源模块中，以便与商用扇形磁质谱仪配合。根据在 10 kV 电位下加速的单电荷质子化离子的离子透过率，将施加到碰撞室和位于碰撞室后面的加速板（左右一对平板）的电压分别优化为 3.0 kV 和 1.5 kV。根据前体离子的强度，该设置允许近 80% 的离子透过率，并且可以提供特定于高能碰撞诱导解离的产物离子。值得一提的是离子采集效率和质量分辨率，尤其是低于质量的分辨率。约前体质量的20%，有了很大的提高。本装置可以使用磁性四扇区仪器从低质量区的MS1中几乎观察不到的初级产物离子实现MS/MS/MS。它将有效地应用于生物分子的结构分析。