High Pressure NMR Study on the Control of Organic Chemical Reaction by Pressure.

压力控制有机化学反应的高压核磁共振研究。

• 批准号: 10640519
• 负责人: YAMADA Hiroaki
• 金额: $ 1.98万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640519/
• 关键词: high pressure NMR; high pressure NMR cell; high pressure quartz cell; paracyclophane; pressure effects; activation volume; pressure-induced molecular deformation

(1).Effect of hydrostatic pressure on the rate of internal rotation of cyclophane derivatives has been examined by DNMR method. Quartz pressure-resisting NMR cells were used to realize the high pressure experiments up to 390 MPa (line shape measurements) and 450 MPa (chemical shift measurements). Application of hydrostatic pressure was found to accelerate the rotation of the benzene ring, while pressure-induced high field chemical shifts of bridge methylene protons indicated that there is a considerable shrinkage of the methylene bridge structure upon pressurization.(2).The technique of using a pressure-resisting cell system for high-pressure high-resolution NMR measurements up to 600 MPa has been accomplished. This system demonstrates capability of performing high-pressure experiments on any standard spectrometer including a modern high field (400-750 MHz) NMR machine. Using this high pressure NMR method, high-pressure studies have been promoted in the field of the high-pressure molecular biology, where performance inherent to the pressure-resisting cell displays its ability to the limit. At present, Kobe University is unrivaled as to this level of experiment.

(1).用差示扫描量热法研究了静水压力对环烷类化合物内旋转速率的影响。利用石英耐压核磁共振池实现了高达390 Mpa(线形测量)和450 Mpa(化学位移测量)的高压实验。施加静水压力可以加速苯环的旋转，而压力引起的高场化学位移的亚甲基质子表明，加压后亚甲基桥结构有相当大的收缩。(2)利用耐压池系统实现了高达600 Mpa的高压高分辨核磁共振测量。该系统展示了在任何标准光谱仪上执行高压实验的能力，包括现代高场(400-750 MHz)核磁共振仪。使用这种高压核磁共振方法，高压研究在高压分子生物学领域得到了推动，耐压细胞固有的性能显示出其能力的极限。目前，神户大学在这方面的实验水平是无与伦比的。

项目成果

Kazuyuki Akasaka: "Pressure response of protein backbone structure.Pressure induced amide ^<15>N chemical shift in BPTI"Protein Science. 8. 1946-1953 (1999)

Kazuyuki Akasaka：“蛋白质主链结构的压力响应。压力诱导BPTI中的酰胺^ 15 N化学位移”蛋白质科学。

K. Akasaka et al.: "Pressure Response of Protein Backbone Structure. Pressure-induced Amide ^<15>N Chemical Shift in BPTI"Protein Science. (in press). (2000)

K.Akasaka等人：“蛋白质主链结构的压力响应。BPTI中压力诱导的酰胺^ 15 N化学位移”蛋白质科学。

H.R. Kalbitzer et al.: "^<15>N and ^1N study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure"Protein Science. (in press). (2000)

H.R. Kalbitzer 等人：“高压下来自肉葡萄球菌的含组氨酸蛋白 (HPr) 的 ^15N 和 ^1N 研究”蛋白质科学。

Kuniaki Itoh: "2,11-Dithia[3.3.1]paracyclophane,(I),and 2,11-Dithia[3.3.2]para-cyclophane,(II)"Acta Cryst.. C55. 1357-1360 (1999)

Kuniaki Itoh：“2,11-Dithia[3.3.1]对环芳烷，(I)，和 2,11-Dithia[3.3.2]对环芳烷，(II)”Acta Cryst.. C55。

H. Li et al.: "Pressure Effect on the Tertiary Structure and Dynamics of Folded BPTI"Biophysical Journal. 77. 2801-2812 (1999)

H. Li 等人：“压力对折叠 BPTI 三级结构和动力学的影响”生物物理学杂志。