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Physiological and biochemical study of D-amino acid-containing peptide, fulicin and fulyal originated from molluscs

软体动物源性含D-氨基酸肽、fulicin、fulyal的生理生化研究

基本信息

批准号：
10640674
负责人：
MINAKATA Hiroyuki
金额：
$ 2.05万
依托单位：
Suntory Institute for Bioorganic Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640674/
关键词：
molluscs neuropeptides in situ hybridization D-amino acid mass spectrometry 分解酵素

项目摘要

(1) Degradation enzyme of fulicin and fulyalAn endopeptidase-like activity was observed when fulicin and fulyal were incubated with the membrane fraction prepared from the subesophageal ganglia of Achatina fulica. The presence of bivalent metal ion and inhibition by specific inhibitors revealed that this activity was attributed to an endopeptidase 24.1.1-like enzyme.(2) Expressions of achatin-I, fulicin, fulyal and ACEP-I (Achatina cardioexcitatory peptide-I)Expressions of precursor cDNAs of achatin-I, fulicin, fulyal and ACEP-I were examined in the central ganglia and the artria of Achatina fulica. Achatin-I cDNA was detected in the subesophageal ganglia, but not in the artria. Precursor cDNAs of fulicin, fulyal, and ACEP-I were observed from both the subesophageal ganglia and the atria. ACEP-I cDNA was also found in the cerebral ganglia. In situ hybridization experiments revealed that achatin-I transcripts were expressed in neurons of pedal ganglia, fulicin and fulyal transcripts were found in those of parietal ganglia, ACEP-I transcripts were detected in those of cerebral and pedal ganglia. From these results, achatin-I was synthesized in neurons of pedal ganglia ; on the other hand fulicin, fulyal, and ACEP-I were produced from both the central ganglia and the atria, and might be artrial hormone-like peptides.(3) Roles of fulicin and fulyal on the reproductive organs of Achatina fulicaFulicin has been thought to control male copulatory behavior in Achatina fulica. The vagina and the oviduct of the snail were densely innervated by fulicin-like immunoreactive neuronal fibers. Fulicin was actually present in the vagina by mass spectrometry analysis. Fulicin showed potent excitatory effect on contractions of the vagina and the oviduct. These results suggest that fulicin controls female egg-laying behavior as an excitatory neuropeptide regulating the female reproductive organs of the snail.

(1)当将Fulicin和Fulyal与从褐云玛瑙螺食道下神经节制备的膜组分孵育时，观察到一种内肽酶样活性。二价金属离子的存在和特异性抑制剂的抑制作用表明，这种活性归因于内肽酶24.1.1样酶。(2)Achatin-I、fulicin、fulyal和ACEP-I（Achatinacardiexcitatorypeptide-I）的表达研究了Achatin-I、fulicin、fulyal和ACEP-I的前体cDNA在褐云玛瑙螺中央神经节和动脉中的表达。Achatin-IcDNA在食管下神经节中检测到，但在动脉中未检测到。从食管下神经节和心房中观察到了富里酸、富里酸和ACEP-I的前体cDNA。脑神经节中也发现了ACEP-I cDNA。原位杂交结果显示，Achatin-I在足神经节表达，Fulicin和Fulyal在顶神经节表达，ACEP-I在大脑和足神经节表达。从这些结果来看，achatin-I在足神经节的神经元中合成;另一方面，fulicin、fulyal和ACEP-I从中央神经节和心房产生，并且可能是心房肽样肽。(3)Fulicin和Fulyal对褐云玛瑙螺生殖器官的作用Fulicin被认为控制着褐云玛瑙螺雄性的交配行为。蜗牛的阴道和输卵管由富里酸样免疫反应阳性神经纤维密集支配。通过质谱分析，Fulicin实际上存在于阴道中。Fulicin对阴道和输卵管的收缩有明显的兴奋作用。这些结果表明，富里菌素作为一种兴奋性神经肽调节雌螺的生殖器官，控制雌螺的产卵行为。