Biomechanics on the mechanical adaptation of biological tissues at the microstructural level

生物力学在微观结构水平上研究生物组织的机械适应

• 批准号: 10650106
• 负责人: YAMAMOTO Noritaka
• 金额: $ 2.18万
• 依托单位: Ritsumeikan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650106/
• 关键词: Biomechanics; Collagen fiber; Tendon; Microstructure; Mechanical properties; Tensile test; Phase-contrast microscope; Differential interference microscope; コラーゲン; 線維; 内側側副靭帯

We developed a new method by which tensile tests were performed observing the microstructure of the chemically non-treated raw collagen fascicles with a phase-contrast microscope or differential interference microscope, and studied the relationships between the microstructure and mechanical properties of fascicles obtained from the mouse tail tendon.For mechanical testing, a specially designed micro tensile tester was mounted on a microscope. Small acrylic blocks were attached to both ends of each fascicle with cyanoacrylate adhesives. One of the blocks was fixed to a load cell, and the other one was fixed to the crosshead of a linear actuator. The fascicle was immersed in a saline solution and sandwiched between a slide glass and cover glass. The specimen was stretched to failure by moving the actuator at the speed of 0.1 mm/sec. The microstructure of the fascicle was observed using the microscope, and the images were recorded on a digital video recorder.Microscopically, the bundles of collagen fibers arranged in a typical parallel fashion were observed in the fascicles. Under no load, the bundles had a periodic crimp pattern. At the liner region of load-deformation curve, the fibers were straightened in parallel. Before the catastrophic decrease of load, the partial rupture of some fibers was observed.

我们开发了一种新方法，通过相差显微镜或微分干涉显微镜观察未经化学处理的原始胶原束的微观结构进行拉伸测试，并研究从小鼠尾腱获得的束的微观结构和力学性能之间的关系。为了进行力学测试，在显微镜上安装了专门设计的微型拉伸测试仪。用氰基丙烯酸酯粘合剂将小的丙烯酸块固定在每个束的两端。其中一个块固定在称重传感器上，另一个固定在线性致动器的十字头上。将分束浸入盐溶液中并夹在载玻片和盖玻片之间。通过以 0.1 毫米/秒的速度移动致动器，将样本拉伸至失效。使用显微镜观察束的微观结构，并将图像记录在数字录像机上。显微镜下，在束中观察到以典型的平行方式排列的胶原纤维束。在无负载的情况下，线束具有周期性卷曲图案。在载荷-变形曲线的线性区域，纤维被平行拉直。在负载发生灾难性下降之前，观察到一些纤维部分断裂。

项目成果

井川英志,山本憲隆: "マウス尾腱とその線維束の力学的関係"日本機械学会関西学生会平成11年度卒業研究発表講演会前刷集. 13-15 (2000)

Hideshi Ikawa、Noritaka Yamamoto：“小鼠尾腱与其纤维束之间的机械关系”日本机械工程学会关西学生会 1999 年毕业研究报告预印本集 13-15（2000）。

E.Ikawa and N.Yamamoto: "Mechanical Relations between Mouse Tail Tendon and Its Fascicles"Proc. JSME Kansai District Student Conference. 13-15 (2000)

E.Ikawa 和 N.Yamamoto：“小鼠尾腱及其束之间的机械关系”Proc。

山本憲隆,他: "マウスより摘出した腱・靭帯の微視的構造と力学的性質" 第11回バイオエンジニアリング講演会講演論文集. (発表予定). (1999)

Noritaka Yamamoto 等人：“从小鼠体内分离出的肌腱和韧带的微观结构和机械特性”第 11 届生物工程会议论文集（待提交）（1999 年）。

M.Tanaka and N.Yamamoto: "Microstructure and Mechanical Properties of the Collagen Fascicles from Mouse Tail Tendon"Proc. 10th JSME Autumn Bioengineering Conference and Seminar. 101-102 (1999)

M.Tanaka 和 N.Yamamoto：“小鼠尾腱胶原蛋白束的微观结构和机械特性”Proc。

井川英志,山本憲隆: "マウス尾腱とその線維束の力学的関係"日本機械学会関西学生会卒業研究発表講演会講演前刷集. (発表予定). (2000)

Hideshi Ikawa、Noritaka Yamamoto：“小鼠尾腱与其纤维束之间的机械关系”日本机械工程学会关西学生会毕业研究报告演讲预印本（待发表）。