Development of Drop-based Visual Test of Hemoglobin in Whole Blood and Its Application to Diagnosis of Anemia

已经发现，在十八烷基硅烷化(ODS)硅板上，一些染料溶质沿着水滴的周长聚集。近年来，分散的微粒通过蒸发形成环的研究在理论和实验上都引起了很大的兴趣。在本研究中，ODS板作为固体表面形成环而不蒸发。将罗丹明B荧光环应用于全血中血红蛋白的目测试验。当一滴稀释的血液和染料放在平板上时，只有在低血红蛋白含量的样品中，才能通过紫外灯在黑暗中观察到产生的荧光环。亲水性蛋白质，即血红蛋白，不会吸附在平板上。它的深红色通过水滴影响荧光环的激发和视觉感受。选择染料浓度来区分分别对应于健康和轻度贫血女性受试者的120g/L和100g/100g/Hb之间的微小差异。在另一项单独的实验中，发现三苯甲烷染料在ODS板上的分配行为随着三个苯环上的取代基的不同而不同。这表明成环是由于所用染料的高疏水性所致。该方法的优点可用于贫血的筛选试验。

It has been found that some dye solutes become concentrated along the perimeter of an aqueous drop on an octadecylsilanized (ODS) silica plate. There has been much theoretical and experimental interest recently in the formation of ring from dispersed micro-particles through evaporation. In this study, ODS plate serves as an solid surface to form a ring without evaporation. The fluorescent ring of Rhodamine B was applied to a visual test of hemoglobin in whole blood. When a drop of diluted blood and the dye is set on the plate, the resulting fluorescent ring can be observed through the drop with an ultraviolet lamp in the dark only for the samples of low hemoglobin content. The hydrophilic protein, hemoglobin, is not adsorbed on the plate. Its deep red color affects the excitation and visual perception of the fluorescent ring through the drop. The dye concentration was chosen to distinguish a slight difference between 120 and 100 g/L hemoglobin, which corresponds to healthy and slightly anemic female subjects, respectively.In a separate experiment, it was found that the partition behavior of the triphenylmethane dyes on an ODS plate varied with the substituted groups on the three phenyl rings. This indicates that the ring formation would be atributed to the high hydrophobicity of the dye used.The advantages of the proposed method can be applied to the screening test of anemia.