Development of Drop-based Visual Test of Hemoglobin in Whole Blood and Its Application to Diagnosis of Anemia

全血血红蛋白滴眼检测法的研制及其在贫血诊断中的应用

• 批准号: 10650792
• 负责人: KANEKO Emiko
• 金额: $ 2.24万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650792/
• 关键词: Whole Blood; Hemoglobin; Rhodamine B; ODS silica; Visual Fluorometry; 目視テスト; ドロップテスト

It has been found that some dye solutes become concentrated along the perimeter of an aqueous drop on an octadecylsilanized (ODS) silica plate. There has been much theoretical and experimental interest recently in the formation of ring from dispersed micro-particles through evaporation. In this study, ODS plate serves as an solid surface to form a ring without evaporation. The fluorescent ring of Rhodamine B was applied to a visual test of hemoglobin in whole blood. When a drop of diluted blood and the dye is set on the plate, the resulting fluorescent ring can be observed through the drop with an ultraviolet lamp in the dark only for the samples of low hemoglobin content. The hydrophilic protein, hemoglobin, is not adsorbed on the plate. Its deep red color affects the excitation and visual perception of the fluorescent ring through the drop. The dye concentration was chosen to distinguish a slight difference between 120 and 100 g/L hemoglobin, which corresponds to healthy and slightly anemic female subjects, respectively.In a separate experiment, it was found that the partition behavior of the triphenylmethane dyes on an ODS plate varied with the substituted groups on the three phenyl rings. This indicates that the ring formation would be atributed to the high hydrophobicity of the dye used.The advantages of the proposed method can be applied to the screening test of anemia.

已经发现，一些染料溶质沿着十八烷基硅烷化（ODS）二氧化硅板上的水滴的周边浓缩。近年来，分散的微粒通过蒸发形成环状结构的研究引起了理论和实验上的广泛兴趣。在本研究中，ODS板作为一个固体表面，形成一个环没有蒸发。将罗丹明B荧光环应用于全血中血红蛋白的目视检测。当将一滴稀释的血液和染料置于平板上时，仅对于血红蛋白含量低的样品，在黑暗中用紫外灯通过该滴可观察到产生的荧光环。亲水性蛋白质血红蛋白不吸附在平板上。其深红色通过液滴影响荧光环的激发和视觉感知。选择染料浓度以区分120和100 g/L血红蛋白之间的轻微差异，这分别对应于健康和轻微贫血的女性受试者。在单独的实验中，发现三苯甲烷染料在ODS板上的分配行为随三个苯环上的取代基而变化。结果表明，成环反应与所用染料的疏水性有关，该方法可用于贫血的筛查。

项目成果

Emiko Kaneko: "Visual Drop Test of Hemoglobin in Whole Blood by Using Ring-like Concentration of Rhodamine B onto Octadecylsilanized Silica Plate"Chemistry Letters. 8. 751-752 (1999)

Emiko Kaneko：“通过在十八烷基硅烷化二氧化硅板上使用罗丹明 B 的环状浓度对全血中的血红蛋白进行视觉滴落测试”化学快报。

Emiko Kaneko: "Visual Drop Test of Hemoglobin in Whole Blood by Using Ring-like Concentration o Rhodamine B onto Octadecylsilanized Silica Plate"Chemistry Letters. 8. 751-752 (1999)

Emiko Kaneko：“通过使用环状浓度 o 罗丹明 B 到十八烷基硅烷化二氧化硅板上对全血中的血红蛋白进行视觉滴落测试”化学快报。

Emiko Kaneko: "Visual Drop Test of Hemoglobin in Whole Blood by Using Ring-like Concentration of Rhodamine B onto Octadecyl-silanized Silica Plate"Chemistry Letters. 751-752 (1999)

Emiko Kaneko：“通过在十八烷基硅烷化二氧化硅板上使用罗丹明 B 的环状浓度对全血中的血红蛋白进行视觉滴落测试”化学快报。