DICARBOXIMIDE RESISTANCE AND OSMOTIC REGULATION IN PLANT PATHOGENS

植物病原体的二甲酰亚胺抗性和渗透调节

• 批准号: 10660052
• 负责人: FUJIMURA Makoto
• 金额: $ 2.3万
• 依托单位: TOYO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660052/
• 关键词: DICARCOXIMIDE; OSMOREGULATION; GLYCEROL; Neurospora; RESISTANCE; 浸透圧; アカパンカビ; 薬剤耐性

Dicarboximides have been applied on various crops against Botrytis cinerea. However, dicarboximide-resistant isolates of B. cinerea appeared on a variety of crops worldwide. To study mode of action and resistant mechanism of dicarboximides we use Neurospora crassa as a model fungus. The osmotic sensitive os-1, os-2, os-4 and os-5 mutants of Neurospora crassa were found to be resistant not only to iprodione but also to fludioxonil. Stimulation of glycerol biosynthesis by these fungicides observed in the wild-type strain was not induced in os mutant strains. The os-1 mutant alleles were classified into two groups based on sensitivity to fungicides and osmotic stress: Type I (highly resistant to fungicides but moderately sensitive to osmotic stress) and Type II (highly sensitive to osmotic stress but moderately resistant to fludioxonil). Sequence analysis of os-1 gene indicate that type I strains were null mutants. Thus we concluded that a os-1 product is essential for these fungicides to express their antifungal activity. Type II mutants have single amino acid substitutions within amino-acid repeats of os-1 gene, suggesting the amino-acid repeats may play a important rule for osmoreguylation.

二甲酰亚胺已被应用于各种作物以对抗灰葡萄孢。然而，B.灰霉病出现在世界各地的各种作物上。以粗糙脉孢菌为模型菌，研究二甲酰亚胺类药物的作用方式和耐药机制。粗糙脉孢菌的渗透敏感突变体os-1、os-2、os-4和os-5不仅对异菌脲而且对咯菌腈都具有抗性。在野生型菌株中观察到的这些杀真菌剂对甘油生物合成的刺激在os突变菌株中没有诱导。根据对杀菌剂和渗透胁迫的敏感性将os-1突变体等位基因分为两组：I型（对杀菌剂高度抗性，但对渗透胁迫中度敏感）和II型（对渗透胁迫高度敏感，但对咯菌腈中度抗性）。os-1基因序列分析表明，Ⅰ型菌株为无效突变株。因此，我们得出结论，os-1的产品是必不可少的，这些杀菌剂表达其抗真菌活性。II型突变体在os-1基因的氨基酸重复序列中有单个氨基酸替换，提示氨基酸重复序列可能在os-1基因的重复序列中起重要作用。

项目成果

M.Fujimura: "Fungicide resistance and Osmotic stress sensitivity in os mutants of Neurospora"Pestic.Biochem,Physiol.. (in press). (2000)

M.Fujimura：“脉孢菌 os 突变体的杀菌剂抗性和渗透胁迫敏感性”Pestic.Biochem，Physiol..（出版中）。

M. Fujumura: "Fungicide resistance and osmotic stress sensitivity in os mutants of Newrospora"Pestec. Biochem. Physiol.. (in press). (2000)

M. Fujumura：“新罗斯孢菌 os 突变体的杀菌剂抗性和渗透胁迫敏感性”Pestec。

M.Fujimura: "Sensitivity to phenylpyrrole fungicides and glycerol accumulation in os and cut Mutant strains of N.crassa"J.Pestic Sci.. 25(1). 31-36 (2000)

M.Fujimura：“对苯基吡咯类杀菌剂的敏感性以及口腔中甘油的积累和粗糙 N.crassa 突变株”J.Pestic Sc​​i.. 25(1)。

M. Fujimura, N. Ochiai, A. Ichiishi, R. Usami, K. Horikoshi and I. Yamaguchi: "Fungicide resistance and osmotic stress sensitivity in os mutants of Neurospora crassa."Pestic. Biochem. Physiol.. 65 (in press). (2000)

M. Fujimura、N. Ochiai、A. Ichiishi、R. Usami、K. Horikoshi 和 I. Yamaguchi：“粗糙脉孢菌 os 突变体的杀菌剂抗性和渗透胁迫敏感性。”害虫。

M. Fujimura, N. Ochiai, A. Ichiishi, R. Usami, K. Horikoshi and I. Yamagushi: "Sensitivity to phenylpryyole fungicides and abnormal glycerol accumulation in os and cut mutant strains of Neurospora crassa."J. Pestic. Sci.. 25 (1). 31-36 (2000)

M. Fujimura、N. Ochiai、A. Ichiishi、R. Usami、K. Horikoshi 和 I. Yamagushi：“对苯基吡咯类杀菌剂的敏感性以及粗糙脉孢菌的口腔和切割突变株中甘油的异常积累。”