Role of stress response signal transduction pathway in trichothecene production in Fusarium graminearum

应激反应信号转导途径在禾谷镰刀菌生产单端孢菌素中的作用

• 批准号: 22580054
• 负责人: FUJIMURA Makoto
• 金额: $ 2.83万
• 依托单位: Toyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580054/
• 关键词: 赤かび病; MAPキナーゼ; cAMP; アカパンカビ; 麦類赤かび病; 殺菌剤; トリコテセン; ストレス応答

The deletion mutants of FgATF1 gene which encodes a putative transcription factor regulated by stress response MAP kinase FgOS2 were isolated and characterized in Fusarium graminearum. Δ FgATF1 strain lack the ability to produce trichothecenes, whereas FgATF1 deletion in an aconidial strain did not affect trichothecene production. These results suggest that FgATF1 indirectly regulates the trichothecene synthesis probably through asexual differentiation. Furthermore we identified 11 genes which are regulated under the stress response MAP kinase pathway, they included various genes such as glycerol dehydrogenase, conidial catalase, clock-controlled genes, gluconeogenesis key enzymes, and cell wall enzymes.

从禾谷镰刀菌中分离到FgATF1基因缺失突变体，该基因编码一个受胁迫反应图激酶FgOS2调控的转录因子。ΔFgATF1菌株缺乏产生三环烯的能力，而非分生菌株的FgATF1缺失不影响三环烯的产生。这些结果表明，FgATF1可能通过无性分化间接调节三氯乙烯的合成。此外，我们还鉴定了11个受胁迫反应图激酶途径调控的基因，包括甘油脱氢酶、分生孢子过氧化氢酶、钟控基因、糖异生关键酶和细胞壁酶等。

项目成果

Comparative transcriptional profiling of Neurospora crassa and Fusarium graminearum in response to fungicides.

粗糙脉孢菌和禾谷镰刀菌对杀菌剂反应的比较转录谱。

• 作者: Makoto Fujimura;Kazuhiro Yamashita;Masakazu Takahashi;Masayuki Kamei;Moto Miyashita
• 通讯作者: Moto Miyashita

Regulation and physiological function of MAP kinase and cAMP-PKA pathways In: Neurospora: Genomics and Molecular Biology.

MAP 激酶和 cAMP-PKA 途径的调节和生理功能见：脉孢菌：基因组学和分子生物学。

• 发表时间: 2013
• 期刊: D. Kasbekar & K. McCluskey (eds).
• 作者: Masayuki Kamei;Masakazu Takahashi;Shinpei Banno;Akihiko Ichiishi;Fumiyasu Fukumori;Makoto Fujimura (2013)
• 通讯作者: Makoto Fujimura (2013)

アカパンカビのストレス応答MAPキナーゼ下流で制御される転写因子RCO-1の同定

粗糙脉孢菌应激反应性 MAP 激酶下游转录因子 RCO-1 的鉴定

• 发表时间: 2010
• 作者: 山下和宏;高橋正和;亀井誠之;藤村真
• 通讯作者: 藤村真

赤かび病菌におけるアカパンカビ薬剤誘導遺伝子の発現解析

镰孢头真菌脉孢菌药物诱导基因的表达分析

• 发表时间: 2011
• 作者: 齋須秀昭;高橋正和;亀井誠之;藤村真
• 通讯作者: 藤村真

Hybridization probe and Pyrosequencing assays to monitor fungicide-resistant population for the cucumber pathogens

杂交探针和焦磷酸测序检测用于监测黄瓜病原体的抗杀菌剂群体

• 作者: Shinpei Banno;Tsuyoshi Shinpuku;Yohei Ishigami;Masakazu Takahashi;Makoto Fujimura
• 通讯作者: Makoto Fujimura