New screenings of the improving factors for lipid metabolism derived from foods and their functional evaluation

食物源性脂质代谢改善因子的新筛选及其功能评价

• 批准号: 10660120
• 负责人: NAGAOKA Satoshi
• 金额: $ 2.11万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660120/
• 关键词: polyphenol; apolipoprotein A-I; cholesterol; genistein; hesperetin; soy peptides; chitosan; rat; リン脂質; Caco-2細胞; ラット; カゼイン; ワイン; 大豆イソフラボン; 動脈硬化; 遺伝子プロモーター

1. (1) In the study about anti-atherogenic factors, effects of polyphenol on apolipoprotein A-I (ApoA-I) gene expression were studied. The results demonstrated that soy genistein increases ApoA-I secretion and its mRNA levels in HepG2 cells. (2) Genistein or hesperetin feeding increses serum ApoA-I and liver ApoA-I mRNA levels in rats.2. In the study about suppressive factors of cholesterol absorption, improving effects for cholesterol metabolism concerning to our soy peptides with bound phospholipids (pepsin peptides or sumizyme peptides) were compared with soy peptides or chitosan which approved by the ministry of health as Food for specified health use. (1) Chitosan feeding decreases body weight gains in rats. Serum and liver cholesterol levels were significantly decreased in soy peptides with bound phospholipids, soy peptides, chitosan-feeding compared with casein feeding. The degree of the cholesterol lowering activity of soy peptides with bound phospholipids was strongest among experimental groups. (2) Cholesterol uptake in Caco-2 cells was decreased by soy peptides with bound phospholipids compared with casein tryptic hydrolysate. Thus, improving effects for cholesterol metabolism of soy peptides with bound phospholipids is superior to those of soy peptides and chitosan which approved by the Ministry of Health and Welfare as Food for specified health use.

1. (1)在抗动脉粥样硬化因子的研究中，研究了多酚对载脂蛋白A-I(ApoA-I)基因表达的影响。结果表明，大豆金雀异黄素可增加 HepG2 细胞中 ApoA-I 的分泌及其 mRNA 水平。 (2)金雀异黄素或橙皮素喂养可增加大鼠血清ApoA-I和肝脏ApoA-I mRNA水平。2．在胆固醇吸收抑制因素的研究中，将本公司的结合磷脂的大豆肽（胃蛋白酶肽或泥酶肽）改善胆固醇代谢的效果与卫生部批准的特定保健用途食品的大豆肽或壳聚糖进行了比较。 (1) 喂食壳聚糖可降低大鼠的体重增加。与酪蛋白喂养相比，结合磷脂的大豆肽、大豆肽、壳聚糖喂养的血清和肝脏胆固醇水平显着降低。结合磷脂的大豆肽降低胆固醇的活性程度在实验组中最强。 (2) 与酪蛋白胰蛋白酶水解产物相比，结合磷脂的大豆肽降低了 Caco-2 细胞中的胆固醇摄取。因此，结合磷脂的大豆肽改善胆固醇代谢的效果优于经厚生省批准为特定保健食品的大豆肽和壳聚糖。

项目成果

Nagaoka, S., Miwa, K., Eto, M., Kuzuya, Y., Hori, G. & Yamamoto, K.: "Soyprotein peptic hydrolyzate with bound phospholipids decrease micellar solubility and cholesterol absorption in rats and Caco-2 cells."Journal of Nutrition. 129(9). 1725-1730 (1999)

长冈，S.，美轮，K.，江藤，M.，葛也，Y.，堀，G.

Nagaoka,S.: "Soyprotein peptic hydrolyzate with bound phospholipids decrease micellar solubility and cholesterol absorption in rats and Caco-2 cells"Journal of Nutrition. 129. 1725-1730 (1999)

Nagaoka,S.：“具有结合磷脂的大豆蛋白消化水解产物降低了大鼠和 Caco-2 细胞中的胶束溶解度和胆固醇吸收”营养学杂志。

Nagaoka S.: "Soyprotein peptic hydrolyzate with bound phospholipids decrease micellar solubility and cholesterol absorption in rats and Caco-2 cells."Journal of Nutrition. 129・9. 1725-1730 (1999)

Nagaoka S.：“结合磷脂的大豆蛋白消化液降低了大鼠和 Caco-2 细胞中的胶束溶解度和胆固醇吸收。”营养学杂志 129・9（1999）。