New screenings of the improving factors for lipid metabolism derived from foods and their functional evaluation

1.(1)在抗动脉粥样硬化因子的研究中，研究了多酚对载脂蛋白A-I(ApoA-I)基因表达的影响。结果表明，大豆金雀异黄素可促进人肝癌细胞ApoA-I的分泌及其mRNA水平。(2)金雀异黄素或橙皮素可增加大鼠血清ApoA-I和肝脏ApoA-Im RNA水平。在对胆固醇吸收抑制因素的研究中，比较了我国大豆结合磷脂多肽(胃蛋白肽或壳聚糖肽)与卫生部批准的大豆多肽或壳聚糖作为特定保健食品对胆固醇代谢的改善作用。(1)喂饲几丁糖可降低大鼠体重增长。与酪蛋白饲料相比，磷脂结合大豆多肽、大豆多肽、壳聚糖可显著降低血清和肝脏胆固醇水平。与磷脂结合的大豆多肽的降胆固醇活性在各试验组中最强。(2)与酪蛋白胰酶水解物相比，结合磷脂的大豆多肽降低了Caco-2细胞对胆固醇的摄取。因此，结合磷脂的大豆多肽改善胆固醇代谢的效果优于卫生福利部批准的特定保健食品中的大豆多肽和壳聚糖。

1. (1) In the study about anti-atherogenic factors, effects of polyphenol on apolipoprotein A-I (ApoA-I) gene expression were studied. The results demonstrated that soy genistein increases ApoA-I secretion and its mRNA levels in HepG2 cells. (2) Genistein or hesperetin feeding increses serum ApoA-I and liver ApoA-I mRNA levels in rats.2. In the study about suppressive factors of cholesterol absorption, improving effects for cholesterol metabolism concerning to our soy peptides with bound phospholipids (pepsin peptides or sumizyme peptides) were compared with soy peptides or chitosan which approved by the ministry of health as Food for specified health use. (1) Chitosan feeding decreases body weight gains in rats. Serum and liver cholesterol levels were significantly decreased in soy peptides with bound phospholipids, soy peptides, chitosan-feeding compared with casein feeding. The degree of the cholesterol lowering activity of soy peptides with bound phospholipids was strongest among experimental groups. (2) Cholesterol uptake in Caco-2 cells was decreased by soy peptides with bound phospholipids compared with casein tryptic hydrolysate. Thus, improving effects for cholesterol metabolism of soy peptides with bound phospholipids is superior to those of soy peptides and chitosan which approved by the Ministry of Health and Welfare as Food for specified health use.