The clarification and application of mechanism of hypocholesterolemic peptides by peptide array

[1]本实验旨在利用多肽阵列技术从大豆蛋白中筛选出具有胆汁酸结合能力的抑制肠道胆固醇吸收的多肽。我们在这次筛选中发现了一些胆汁酸结合肽。此外，我们还评价了多肽阵列对胆固醇吸收抑制肽(VAWWMY)活性的有效修饰。我们发现PWWWMY和VIWWFK是比VAWWMY具有更高活性的新的多肽。[2]我们用荧光素酶启动子实验研究了胆固醇7α-羟基酶基因反式激活的机制。我们发现HNF3或HNF4被掺入到Lactostatin诱导的HepG2细胞中CYP7A1基因的反式激活。

[1] This experiment was designed to identify the peptides that have bile acid-binding ability from soybean protein to inhibit an intestinal cholesterol absorption by peptide array. We found some bile acid binding peptides in this screening. Furthermore, we evaluated the efficient modification of VAWWMY (cholesterol absorption inhibitory peptide) activity by peptide array. We found PWWWMY and VIWWFK as new peptides with higher activity than VAWWMY.[2] We studied the mechanism of cholesterol 7α-hydroxylase (CYP7A1) gene transactivation by a luciferase promoter assay. We found that HNF3 or HNF4 were incorporated into the lactostatin induced the transactivation of CYP7A1 gene in HepG2 cells.