Imprint of embryonic stage by color light exposure and it application

1. 鹌鹑雏鸡视网膜形态计量学研究：孵化10天后，观察到10个视网膜亚层。孵育第12天，各层厚度发育良好，视网膜厚度显著增加。培养第13天，颗粒层出现油滴。当时的油滴还没有颜色，大小为1.5微米。培养15天后，油滴有颜色；红色、黄色、蓝色、绿色和透明。胚胎孵化时，油滴大小为3微米。每种油滴在胚胎各阶段的比例为1（黄色）：1（红色）：1（绿色）：2（透明）。胚胎视网膜组织化学研究：胚胎视网膜第10天未见胺能神经元。第13天可见胺能神经元。在此期间，神经元每周进行染色，就像染色一样。第14 ~ 15天，胚胎视网膜上可见明显的含胺神经元及其突起。胺能神经元的大小也在变大。视网膜平片显示，胺能神经元树突呈放射状分布。色光照射后的行为研究。色光；绿、红、黄三色为孵化第13天胚在蛋壳上打好孔后的颜色。孵化后进行颜色偏好测试。对照组以绿色为主。暴露在黄色和绿色光下的组更喜欢黄色。结果表明，雏鸡在胚胎期就有对颜色的偏好。

1. Morphometric study of retina of quail chick : ten retinal sublayers were seen at ten days of incubation. At 12 th days in incubation, thickness of each layer was developed well and thickness of retina was increased dramatically. Oil droplet was seen in granular layer at incubation 13 th days. In that time oil droplet has not color and the size of it was 1.5 micron meter. After incubation 15 th, oil droplet had color ; red, yellow, blue, green and clear. The size of oil droplet was three micron meter at embryo was hatched. Proportion of each kind of oil droplet was 1(yellow): 1(red): 1(green): 2(clear) in every stage of embryo.2. Histochemical study of embryonic retina : At 10 th days of embryonic retina, aminergic neurons were not seen. Aminergic neuron was seen at 13 th days of incubation. In that time, neurons were stained weekly just like getting stain. From 14 th to 15 th days of incubation, embryonic retina had evidently seen as amin contained neurons and their processus were seen. The size of aminergic neurons also getting large. In flat mount of retinal specimen showed that the dendrites of aminergic neurons run radially.3. Behavior study after exposed color lights. Color light ; green, red and yellow, was given to the 13 th days incubated embryo after well was made on the egg shell. After they hatched, color preference test was examined. Control group was prefer green. Group which exposed to yellow and green lights prefers yellow. Result showed that there is chick tend to like color which was exposed in embryonic stage.