Imprint of embryonic stage by color light exposure and it application

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1. Morphometric study of retina of quail chick : ten retinal sublayers were seen at ten days of incubation. At 12 th days in incubation, thickness of each layer was developed well and thickness of retina was increased dramatically. Oil droplet was seen in granular layer at incubation 13 th days. In that time oil droplet has not color and the size of it was 1.5 micron meter. After incubation 15 th, oil droplet had color ; red, yellow, blue, green and clear. The size of oil droplet was three micron meter at embryo was hatched. Proportion of each kind of oil droplet was 1(yellow): 1(red): 1(green): 2(clear) in every stage of embryo.2. Histochemical study of embryonic retina : At 10 th days of embryonic retina, aminergic neurons were not seen. Aminergic neuron was seen at 13 th days of incubation. In that time, neurons were stained weekly just like getting stain. From 14 th to 15 th days of incubation, embryonic retina had evidently seen as amin contained neurons and their processus were seen. The size of aminergic neurons also getting large. In flat mount of retinal specimen showed that the dendrites of aminergic neurons run radially.3. Behavior study after exposed color lights. Color light ; green, red and yellow, was given to the 13 th days incubated embryo after well was made on the egg shell. After they hatched, color preference test was examined. Control group was prefer green. Group which exposed to yellow and green lights prefers yellow. Result showed that there is chick tend to like color which was exposed in embryonic stage.