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Morophological analysis of the c-Kit expressing cells that control gastrointestinal motility

控制胃肠运动的 c-Kit 表达细胞的形态学分析

基本信息

批准号：
10670020
负责人：
NAKAMURA Kei-ichiro
金额：
$ 2.37万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670020/
关键词：
gastrointestinal motility pacemaker c-Kit gap junctions mast cells Interstitial cells of Cajal W mutant mice モルモット Interstitial cells of Caial W mutant マウス

项目摘要

1. We identified connexin45-expressing cells that form a cellular network in the deep muscular plexus layer of dog and rat small intestine by immunohistochemistry. By comparing guinea pig c-Kit positive cellular network, functional properties of connexin45 expressing cells are considered as a conduction system in the intestinal wall.2. Connexin43 expressing cells were also identified in the submuscular plexus of dog and guinea pig colon. Gap junctions were observed under electron microscopy between same type of cells and with surrounding smooth muscle cells.3. We observed spindle-shaped c-Kit immunopositive cells within circular muscle layer of guinea pigs and identified those cells were mast cells under electron microscopy.4. By immunohistochemistry, cells positive for both c-Kit and CD34 and positive singly for CD34 were identified in mouse colon muscular layer.5. By comparing W/WィイD1VィエD1 mutant and wild type mice, c-Kit positive cells within circular muscle layer of colon were found to be eliminated specifically in the mutant animals.Taken together, we conclude that c-Kit positive cells in deep muscular plexus form cellular network connected via connexin45 and work as a conduction system and interstitial cells of signal transmission between enteric nervous system and smooth muscle cells, and that those that form network in submuscular plexus of colon are connected via connexin43 gap junctions to form conduction system and pacemaker together with c-Kit positive cells within circular layer.We are planning experiments that clarify distribution and organization of specific cells type that are connected specific type of connexin molecules by in situ hybridization and by producing knockout mice of the specific connexin molecules in order to understand the mechanisms that regulate whole tissue motility other than enteric nervous system.

1.我们通过免疫组化鉴定了表达连接蛋白45的细胞，它们在狗和大鼠小肠的深肌丛层中形成细胞网络。通过比较豚鼠c-Kit阳性细胞网络，认为表达connexin 45的细胞的功能特性是肠壁的传导系统.在狗和豚鼠结肠的肌下神经丛中也鉴定了表达连接蛋白43的细胞。电镜下观察到同种细胞之间及与周围平滑肌细胞之间存在缝隙连接.电镜下观察到豚鼠环形肌层内有梭形c-Kit免疫阳性细胞，经鉴定为肥大细胞.免疫组化结果显示，小鼠结肠肌层中存在c-Kit和CD 34双阳性细胞和CD 34单阳性细胞.通过比较W/W大肠杆菌D1 V大肠杆菌D1突变体和野生型小鼠结肠环肌层内c-Kit阳性细胞，发现突变体小鼠结肠环肌层内c-Kit阳性细胞被特异性清除，说明深肌丛内c-Kit阳性细胞通过连接蛋白45形成细胞网络，作为肠神经系统和平滑肌细胞之间信号传递的传导系统和间质细胞发挥作用。在结肠肌下神经丛中形成网络的神经元通过缝隙连接蛋白43（connexin 43 gap junctions）与c-我们正在计划通过原位杂交和通过产生特定连接蛋白分子的敲除小鼠来阐明连接特定类型连接蛋白分子的特定细胞类型的分布和组织的实验，以便了解调节整个组织运动的机制，而不是肠神经系统。