Morophological analysis of the c-Kit expressing cells that control gastrointestinal motility

1.我们识别的连接45-通过免疫病理化学来表达一个细胞，该细胞形成了一个细胞网络，形成了一个深度肌肉复合体层的狗和大鼠小意图。通过比较几内亚猪C-Kit阳性蜂窝网络，连接45表达细胞的功能属性被认为是一个在intestinal wall中的行为系统。Connexin 43表示细胞也被识别为狗和几内亚猪冒号的亚肌肉表现。间隙交叉在相同类型的细胞和环绕光滑的肌肉细胞下观察到电子显微镜。我们观察到的螺旋形c-Kit免疫细胞在几内亚猪的圆形肌肉层和识别的那些细胞是电子显微镜下的大细胞。根据免疫病理学，细胞对C-Kit和CD 34都有阳性，CD 34在小鼠结肠肌肉层中也有阳性。通过比较W/W D1 V D1突变型和野生型老鼠，在大肠杆菌层中发现的圆形肌肉层被排除在突变动物中的特殊细胞中，我们将c-Kit阳性细胞结合在一起，通过连接45和工作作为一个传导系统和信号传输之间的中间细胞，结合在一起，我们将c-Kit阳性细胞在深层肌肉表现形式的蜂窝网络中连接，作为一个传导系统和内部神经系统和光滑肌肉细胞之间的信号传输的中间细胞，而且，这些表格网络中的冒号的亚肌肉表现形式是通过连接43个间隙连接到表格行为系统和起搏器与C-连接的。在圆形层中的正细胞，我们正在规划一个实验，即分类分布和特定细胞类型的组织，该实验是通过原位杂交连接的特定类型的连接分子，并通过按顺序生产特定连接分子的鼠标。理解一种机制，即规范整个组织的动机高于实体神经系统。

1. We identified connexin45-expressing cells that form a cellular network in the deep muscular plexus layer of dog and rat small intestine by immunohistochemistry. By comparing guinea pig c-Kit positive cellular network, functional properties of connexin45 expressing cells are considered as a conduction system in the intestinal wall.2. Connexin43 expressing cells were also identified in the submuscular plexus of dog and guinea pig colon. Gap junctions were observed under electron microscopy between same type of cells and with surrounding smooth muscle cells.3. We observed spindle-shaped c-Kit immunopositive cells within circular muscle layer of guinea pigs and identified those cells were mast cells under electron microscopy.4. By immunohistochemistry, cells positive for both c-Kit and CD34 and positive singly for CD34 were identified in mouse colon muscular layer.5. By comparing W/WィイD1VィエD1 mutant and wild type mice, c-Kit positive cells within circular muscle layer of colon were found to be eliminated specifically in the mutant animals.Taken together, we conclude that c-Kit positive cells in deep muscular plexus form cellular network connected via connexin45 and work as a conduction system and interstitial cells of signal transmission between enteric nervous system and smooth muscle cells, and that those that form network in submuscular plexus of colon are connected via connexin43 gap junctions to form conduction system and pacemaker together with c-Kit positive cells within circular layer.We are planning experiments that clarify distribution and organization of specific cells type that are connected specific type of connexin molecules by in situ hybridization and by producing knockout mice of the specific connexin molecules in order to understand the mechanisms that regulate whole tissue motility other than enteric nervous system.