Mechanisms of non-quantal amino acid release by nitric oxide

一氧化氮释放非量子氨基酸的机制

• 批准号: 10670081
• 负责人: YOSHIOKA Koichi
• 金额: $ 1.98万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670081/
• 关键词: neurotransmitters; nitric oxide; amino acids; transporters

The mechanisms of actions of substance P and nitric oxide (NO) and associated substances on the release of amino acids from neonatal rat spinal cord preparations were examined and the possibility of involvement of amino acid transporters in the release was explored.Application of substance P (10 μM) by perfusion to the spinal cord induced a release of glutamate, aspartate, GABA, glycine and taurine. The NO donor, SNAP (300 μM) and SIN-1 (300 μM) also released these amino acids in a similar manner. The amino acid release evoked by substance P did not change in the presence of calcium-free medium. However, perfusion of a low-Na medium significantly reduced the amino acid release. The release of GABA, glycine and taurine was reduced by perfusion of a low-Cl medium. The substance P-evoked release of GABA was also suppressed by an inhibitor of GABA transporter. Similar findings were found in the amino acid release evoked by the NO donors.These results suggest that substance P as well as NO and its donors evoke a release of amino acids from the neonatal rat spinal cord and reversal operation of transporters of amino acids is involved in the mechanism of release.

本文研究了P物质和一氧化氮(NO)及其相关物质对新生大鼠脊髓组织中氨基酸释放的作用机制，并探讨了氨基酸转运体参与这一过程的可能性。P物质(10μM)灌流脊髓可诱导谷氨酸、天冬氨酸、氨基丁酸、甘氨酸和牛磺酸的释放。NO供体SNAP(300μM)和SIN-1(300μM)也以类似的方式释放这些氨基酸。无钙条件下，P物质引起的氨基酸释放无明显变化。然而，低钠介质的灌流显著减少了氨基酸的释放。灌流低氯介质可减少GABA、甘氨酸和牛磺酸的释放。由P物质引起的GABA释放也被GABA转运体抑制剂抑制。在NO供体引起的氨基酸释放中也发现了类似的结果。这些结果表明，P物质和NO及其供体引起新生大鼠脊髓氨基酸的释放，氨基酸转运体的逆转操作参与了这种释放的机制。

项目成果

M. Abe et al.: "Drug sensitivities of the primary afferents and their changes after inflammation"Clinical Orthopaedics and Related Research. 369. 340-438 (1999)

M. Abe 等：“主要传入神经的药物敏感性及其炎症后的变化”临床骨科及相关研究。

M. Abe et al.: "Drug sensitivities of the primary Afferents and their changes after inflammation"Clinical Orthopaedics and Related Research. 369. 340-348 (1999)

M. Abe 等：“主要传入神经的药物敏感性及其炎症后的变化”临床骨科及相关研究。

吉岡耕一: "ブレインサイエンスレビュー1999"医学書院. 222 (1999)

吉冈浩一：“脑科学评论 1999” Igaku Shoin 222 (1999)。

H.Suzuki et al.: "Differential effects of wortmannin on the release of substance P and amino acids from the isolated spinal cord of the neonatal rat." British Journal of Pharmacology. 125. 1661-1668 (1998)

H.Suzuki 等人：“渥曼青霉素对新生大鼠离体脊髓释放 P 物质和氨基酸的不同影响。”

M.Abe et al.: "Drug sensitivities of the primary afferents and their changes after inflammation"Clinical Orthopaedics and Related Research. 369. 340-348 (1999)

M.Abe 等：“主要传入神经的药物敏感性及其炎症后的变化”临床骨科及相关研究。