Molecular pathology of stomach and esophagus carcinogenesis induced br duodenal reflux containing bile - a concern of UACL (ulcer-associated cell lineage) to carcinogenesis

胃和食管癌发生的分子病理学诱导含胆汁的十二指肠反流 - UACL（溃疡相关细胞谱系）对癌发生的关注

• 批准号: 10670201
• 负责人: HATTORI Takanori
• 金额: $ 1.86万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670201/
• 关键词: stomach; carcinogenesis; bile reflux; duodenal juice reflux; gastritis cystica profunds; UACL; trefoil peptide; pS2; 胃癌; 残胃癌; 胆汁; 十二指腸液; パレット食道

The current study was undertaken how duodenal contents cause a mucosal damage leading to carcinogenesis. In recent years, attentions have been focused on expression of trefoil peptides in the normal and damaged gastrointestinal mucosa, whereas little was known about their function. In this study, a special attention has been focused on the function of the peptides, and an implication of trefoil peptides expression in the reflux carcinogenesis was studied. Research results, were, (1) by the experiments using human colonic and gastric cancer cell lines, one of trefoil peptides TFF3 has a function of controlling the expression of the APC-catenin and the E-cadherin-catenin complexes, thereby indicating the regulatory functions of cell proliferation related to movements and anchoring of epithelial cells, and (2) by the reflux operation, gastric and esophageal cancers could be induced after 75 weeks. Before 75 weeks, precancerous lesions were studies. In all rats, cystic proliferation of mucous glands, identical with those reported by Wright as UACL, and UACLs expressed pS2 and TFF3, and they were shown to become carcinoma cells.

目前的研究是如何进行的十二指肠内容物引起粘膜损伤，导致癌变。近年来，三叶肽在正常和损伤的胃肠道黏膜中的表达受到广泛关注，但其功能尚不清楚。在这项研究中，一个特别的注意力集中在肽的功能，三叶肽表达的反流癌变的影响进行了研究。研究结果是，（1）通过使用人结肠癌和胃癌细胞系的实验，三叶肽TFF 3之一具有控制APC-连环蛋白和E-钙粘蛋白-连环蛋白复合物的表达的功能，从而表明与上皮细胞的运动和锚定相关的细胞增殖的调节功能，和（2）通过回流操作，75周后可诱发胃癌和食管癌。在75周前，研究癌前病变。在所有大鼠中，粘液腺的囊性增生，与Wright报道的UACL相同，并且UACL表达pS2和TFF 3，并且它们显示为癌细胞。

项目成果

Shiomi, H. et al.: "Cellular distribution and clinical value of urokinase-type plasminogen activator, its receptor, and plasminogen activator inhibitor-2 in esophageal squamous cell carcinoma"Amer. J. Pathol.. 156. 565-575 (2000)

Shiomi, H. 等人：“食管鳞状细胞癌中尿激酶型纤溶酶原激活剂、其受体和纤溶酶原激活剂抑制剂 2 的细胞分布和临床价值”Amer。

Ikehara, Y., et al.: "The aberrant expressional of Lewis a antigen in intestinal metaplastic cells of gastric mucosa is caused by augmentation of Lewis enzyme expression"Glycoconjugate Journal. 15. 799-807 (1998)

Ikehara, Y.等人：“胃粘膜肠化生细胞中Lewis抗原的异常表达是由Lewis酶表达增强引起的”糖缀合物杂志。

Wu, LB. et al.: "Intramucosal carcinomas of the stomach : phenotypic expression and loss of heterozygosity at microsatellites like linked to the APC Gene"Pathol Res. Pract.. 194. 405-412 (1998)

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Ikehara, Y. et al.: "The aberrant expression of Lewis a antigen in intestinal metaplastic cells of gastric mucosa is caused by augmentation of Lewis enzyme expression"Glycoconjugate journal. 15. 799-807 (1998)

Ikehara，Y.等人：“胃粘膜肠化生细胞中Lewis抗原的异常表达是由Lewis酶表达增强引起的”Glycoconjugate杂志。

Yao, XH., et al.: "Regulation of apoptotic cell death in the pyloric glands of the canine stomach"Virchows Arch. 433. 275-280 (1998)

Yao, XH., et al.：“犬胃幽门腺细胞凋亡的调节”Virchows Arch。