A study on the chemotaxis of the larvae of Strongyloides ratti

鼠类圆线虫幼虫趋化性的研究

• 批准号: 10670231
• 负责人: TADA Isao
• 金额: $ 1.86万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670231/
• 关键词: Strongyloides ratti; chemotaxis; third-stage larva; amphid; thermotaxis; enzyme suppression; 化学走行性; ナトリウムイオン; レセプター阻害; β-グルコシダーゼ

Aiming at the clarification of chemotaxis of Strongyloides ratti, a murine nematode parasite, we exaimined the behavior of the third stage larvae (L3) on an agarose plate adjusted with various concentrations of NaCl. The results obtained are as follows : 1) L3 were inhibited their movements at 500 mM or higher in NaCl. 2) L3 showed escaping behavior to lower concentration area in 80-500mM. 3) L3 moved freely in 80 mM or less, while they did not cross the line of 80 mM to higher zone. The movement was shown to be quite sharp in recognition of 80 mM NaCl concentration. The sensitivity thus shown on agarose plates was inhibited by various chemicals and enzymes such as β-glycosidase, β-galactosidase, hyaluronidase, trypsin, lipase, protease, phospholypase, soybean agglutinin, wheat germ agglutinin, spermidine. On this context, we examined the effects of some enzymes on the thermotaxis of this nematode. Hyaluronidase and β-Glucuronidase glucuronidase inhibited this sensitivity of L3 on agaroas. In vivo movements of L3, however, percutaneously inoculated into rats was not disturbed in the course of migration. Present study suggested that mucopolysaccharides derived from hyaluronic acid may play important roles in the amphids of the nematode movements.

为阐明鼠源线虫的趋化性，在琼脂糖平板上观察了鼠源线虫幼虫的趋化行为。结果表明：（1）在500 mM或更高浓度的NaCl溶液中，L3的运动受到抑制。2)L3在80- 500 mM范围内表现出向低浓度区逃逸的行为。3)L3在80 mM或更低浓度下自由移动，而它们没有越过80 mM的线到更高的区域。在80 mM NaCl浓度下，该运动显示出相当尖锐的识别。琼脂糖平板上显示的敏感性可被各种化学品和酶抑制，如β-糖苷酶、β-半乳糖苷酶、透明质酸酶、胰蛋白酶、脂肪酶、蛋白酶、磷脂酶、大豆凝集素、麦胚凝集素、亚精胺。在此背景下，我们研究了一些酶对这种线虫的趋热性的影响。Hyp和β-Glucuronidase可抑制L3对琼脂的这种敏感性。然而，在L3的体内运动中，接种到大鼠中的Perceptin在迁移过程中不受干扰。本研究提示透明质酸来源的粘多糖可能在线虫运动的端头体中起重要作用。