ACTIVATION AND QPOPTOSIS IN T LYMPHOCYTES INDUCED BY NEMATODE ANTIGENS

线虫抗原诱导 T 淋巴细胞的激活和 Qpoptosis

• 批准号: 10670235
• 负责人: UCHIKAWA Ryuichi
• 金额: $ 0.38万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670235/
• 关键词: nematode antigens; T lymphocytes; activation; apoptosis; Nippostrongylus brasiliensis; アポトーシス

In this project (1998-1999), we pointed out the followings ;1. The numbers of total mesenteric lymph node (MLN) cells increased by 14-hold 2 weeks after infection with the nematode Nippostrongylus brasiliensis. Although both CD4ィイD1+ィエD1, and CD8ィイD1+ィエD1 T cells showed 9-10-fold increases in number, the CD4ィイD1+ィエD1/CD8ィイD1+ィエD1 T cell ratios also increased after the infection indicating more obvious activation in CD4ィイD1+ィエD1 T cells.2. Both CD4ィイD1+ィエD1 and CD8ィイD1+ィエD1 T cells from naive rats expressed mRNA for Th1 type cytokines, IL-2, IFN-γ and TNF-β, but not Th2 cytokines such as IL-3, Il-4, Il-5 and IL-13.3. CD4ィイD1+ィエD1 T cells from infected rats markedly increased IL-3, IL-4, IL-5 and IL-13 mRNA expression. Infected CD8ィイD1+ィエD1 T cells did not expressed IL-4 mRNA, although IL-3, IL-5 and IL-13 gene expressions were enhanced.4. Nematode infection increased the numbers CD4ィイD1+ィエD1 T cells in MLN of LEC mutant rats having helper T cell immunodeficiency. The expansion of CD4ィイD1+ィエD1 T cells consisted with marked increase of IL-4 mRNA expression in MLN cells and induced following Th2 responses such as elevation of total serum IgE and mastocytosis in the small intestine and lungs.5. Excretory-secretory (ES) antigen derived from the nematode suppressed transcription and production of IFN-γ by CD4ィイD1+ィエD1 T cells from naive rats during polyclonal stimulation with anti-CD3 plus anti-CD28 antibodies in dose dependent manner.6. Nematode infection induced villus atrophy and apoptosis and ES antigen was detected in the regions.7. ES antigen did not induced apoptosis in CD4ィイD1+ィエD1 and CD8ィイD1+ィエD1 T cells in vitro.

在这个项目（1998-1999）中，我们指出了以下几点：1。总肠系膜淋巴结（MLN）细胞的数量增加了14-hold感染后2周与线虫Nippostrongylus brasiliensis。虽然CD 4 + CD 4 + D1和CD 8 + D1+ CD 4 + D1 T细胞的数量均显示出9-10倍的增加，但CD 4 + D1+ CD 4 + D1/CD 8 + D1+ CD 4 + D1 T细胞的比例也在感染后增加，表明CD 4 + D1+ CD 4 + D1 T细胞的活化更明显.来自幼稚大鼠的CD 4 + CD 4 + D1 + CD 8 + D1+ CD 8 + D1 T细胞均表达Th 1型细胞因子IL-2、IFN-γ和TNF-β的mRNA，但不表达Th 2型细胞因子如IL-3、IL-4、IL-5和IL-13。感染大鼠的CD 4 + CD 4 + CD 4 + D1 + T细胞显著增加IL-3、IL-4、IL-5和IL-13 mRNA表达。感染后的CD 8 + CD 8 + CD 8 + CD 8 + D1 T细胞不表达IL-4 mRNA，但IL-3、IL-5和IL-13基因表达增强.线虫感染可增加辅助性T细胞免疫缺陷LEC突变大鼠MLN中CD 4 + CD 4 + D1 + T细胞的数量。CD 4 + CD 4 + CD 4 + D1+ T细胞的扩增与MLN细胞中IL-4 mRNA表达的显著增加相一致，并诱导了Th 2应答，如血清总IgE升高和小肠和肺中的肥大细胞增多症.来源于线虫的排泄-分泌（ES）抗原以剂量依赖性方式抑制在用抗-CD 3加抗-CD 28抗体的多克隆刺激期间来自幼稚大鼠的CD 4 + CD 4 + D1+ T细胞IFN-γ的转录和产生。线虫感染引起绒毛萎缩和细胞凋亡，并在该区域检测到ES抗原. ES抗原在体外对CD 4 + CD 4 + D1和CD 8 + D1+ CD 8 + D1 T细胞无诱导凋亡作用。

项目成果

Hyoh,Y.: "Enhancement of apoptosis with loss of cellular adherence in the villas epithelium of the small intestine after infecation with the rematode Nippostrongylus brasiliensis"Parasitology. 119. 199-207 (1999)

Hyoh，Y.：“感染巴西日圆线虫后小肠别墅上皮细胞凋亡增强，细胞粘附丧失”寄生虫学。

Uchikawa, R: "Th2cell-associated immune respenses induced by Nippostrongylus brasiliensis infection in LEC"The Proceedings of the 9th International Congress of Paresitalogy. 1998. 551-555 (1998)

Uchikawa, R：“LEC 中巴西圆线虫感染诱导的 Th2 细胞相关免疫反应”第九届国际轻瘫学大会论文集。

Uchikawa R, Matsuda S, Yamada M, Nishida M, Agui T and Arizono N: "Th2 cell-associated immune responses induced by Nippostrongylus brasiliensis infection in LEC mutant rats with helper T cell immunodeficiency"The Proceedings of the 9th International Congr

Uchikawa R、Matsuda S、Yamada M、Nishida M、Agui T 和 Arizono N：“辅助 T 细胞免疫缺陷的 LEC 突变大鼠中由巴西圆线虫感染诱导的 Th2 细胞相关免疫反应”第九届国际会议论文集