ROLES OF NEMATODE ANTIGENS ON TH2 TYPE T CELL INDUCTION IN RATS.

线虫抗原对大鼠 TH2 型 T 细胞诱导的作用。

• 批准号: 08670285
• 负责人: UCHIKAWA Ryuichi
• 金额: $ 1.41万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670285/
• 关键词: TH2 CELL; RAT; Nippostrongylus brasiliensis; ES ANTIGENS; CYTOKINE; NEMATODE; IL-4; INTERFERON-gamma; Nippostrmgylua brasiliensis; インターフェロン-ガンマ; nematode; rat; Th2; T all; mRNA

In this project (1996-1997), we pointed out the followings ;1.When less than 1.0mug/ml of ES antigens derived from Nippostrongylus brasiliensis (Nb) adult worms, IL-4 production by rat MLN cells increased, while higher concentrations (more than 10.0mug/ml) suppressed IL-4 and IFN-gamma productions in a dose dependent manner.2.Preincubation of MLN cells with ES antigens (10mug/ml) for 3-hours inhibited further IFN-gamma production by Con A stimulated MLN cells.3.Es antigens did mot affect IFN-gamma production of MLN cells stimulated by immobilized anti-CD3 monoclonal antibody.4.CD4^+T cells derived from Nb infected rats showed increased IL-3,4,5 mRNA expressions. CD8^+T cells also revealed increased levels of IL-3,5 mRNA,but not IL-4.5.Con A stimulated CD8^+T cells produced two times IFN-gamma as much as CD4^+T cells did. Suppression of IFN-gamma production of CD8^+T cells observed when more than 1.0mug/ml of ES antigens were added, while the production of CD4^+T cells was suppressed by more than 10.0mug/ml of ES antigens.6.Nb infection was able to induce Th2 associated immune responses in LEC rats with helper T cell immunodeficiency.7.Partially purification of Nb adult worm antigens was performed using an ion exchange chromatography. Then we found a fraction that increased IL-4 and decreased IFN-gamma production by Con A stimulated MLN cells.

在这个项目中（1996-1997），我们指出：1.当来自巴西日本圆线虫（Nb）蠕虫的ES抗原浓度低于1.0 μ g/ml时，大鼠MLN细胞产生IL-4的能力增加，而更高浓度的（大于10.0 μ g/ml）以剂量依赖方式抑制IL-4和IFN-γ的产生（10 μ g/ml）作用3小时，抑制ConA刺激MLN细胞产生IFN-γ;（3）Es抗原对固定化抗CD 3单克隆抗体刺激MLN细胞产生IFN-γ无影响;（4）Nb感染大鼠CD 4 ^+T细胞IL-3，4，5 mRNA表达增加。CD 8 ^+T细胞也显示IL-3.5 mRNA水平增加，但IL-4.5水平没有增加。Con A刺激的CD 8 ^+T细胞产生的IFN-γ是CD 4 ^+T细胞的两倍。当加入超过1.0 μ g/ml的ES抗原时，观察到CD 8 ^+T细胞IFN-γ的产生受到抑制，而CD 4 ^+T细胞的产生被抑制超过10.0 μ g/ml。6. Nb感染可诱导辅助性T细胞免疫缺陷的LEC大鼠产生Th 2相关的免疫应答层析然后，我们发现了一个馏分，增加IL-4和减少IFN-γ的生产由刀豆蛋白A刺激MLN细胞。

项目成果

UCHIKAWA,Ryuichi: "Nematode infection in duces Th2 cell-associated immune nesponses in LEC mutant rats with helper T cell immurodeficiency" Parasite Immunology. 19. 461-468 (1997)

UCHIKAWA，Ryuichi：“线虫感染在辅助性 T 细胞免疫缺陷的 LEC 突变大鼠中诱导 Th2 细胞相关的免疫反应”寄生虫免疫学。