ROLES OF NEMATODE ANTIGENS ON TH2 TYPE T CELL INDUCTION IN RATS.

在这个项目(1996-1997)中，我们指出了以下几点；1.当巴西拟青虫成虫ES抗原浓度低于1.0mug/ml时，大鼠髓鞘细胞产生的IL-4增加，而高浓度(>10.0mug/ml)则抑制IL-4和干扰素-γ的产生，且呈剂量依赖关系。2.与ES抗原(10mug/ml)共孵育3h，可进一步抑制ConA刺激的MLN细胞产生的干扰素-γ。3.ES抗原对固定化抗CD3单抗刺激的MLN细胞产生的干扰素-γ无明显影响。4.来自NB感染大鼠的CD4+T细胞表现出IL-3、4、4、4的增加。5mRNA的表达。CD8+T细胞产生的IL-3、5mRNA水平增加，而IL-4.5水平无明显变化。Con A刺激的CD8+T细胞产生的干扰素是CD4+T细胞的两倍。当ES抗原浓度大于1.0mug/ml时，CD8^+T细胞产生的干扰素-γ受到抑制，而当浓度超过10.0mug/ml时，CD8^+T细胞的产生受到抑制。6.在辅助性T细胞免疫缺陷的LEC中，Nb感染能够诱导Th2相关的免疫应答。然后，我们发现了一个组分，通过ConA刺激的MLN细胞增加IL-4，减少干扰素-γ的产生。

In this project (1996-1997), we pointed out the followings ;1.When less than 1.0mug/ml of ES antigens derived from Nippostrongylus brasiliensis (Nb) adult worms, IL-4 production by rat MLN cells increased, while higher concentrations (more than 10.0mug/ml) suppressed IL-4 and IFN-gamma productions in a dose dependent manner.2.Preincubation of MLN cells with ES antigens (10mug/ml) for 3-hours inhibited further IFN-gamma production by Con A stimulated MLN cells.3.Es antigens did mot affect IFN-gamma production of MLN cells stimulated by immobilized anti-CD3 monoclonal antibody.4.CD4^+T cells derived from Nb infected rats showed increased IL-3,4,5 mRNA expressions. CD8^+T cells also revealed increased levels of IL-3,5 mRNA,but not IL-4.5.Con A stimulated CD8^+T cells produced two times IFN-gamma as much as CD4^+T cells did. Suppression of IFN-gamma production of CD8^+T cells observed when more than 1.0mug/ml of ES antigens were added, while the production of CD4^+T cells was suppressed by more than 10.0mug/ml of ES antigens.6.Nb infection was able to induce Th2 associated immune responses in LEC rats with helper T cell immunodeficiency.7.Partially purification of Nb adult worm antigens was performed using an ion exchange chromatography. Then we found a fraction that increased IL-4 and decreased IFN-gamma production by Con A stimulated MLN cells.