T CELL FUNCTION FOR IgE ANTIBODY PRODUCTION IN NEMATODE INFECTED RAT AND EFFECTS OF NEMATODE ANTIGENS.

线虫感染大鼠中 T 细胞产生 IgE 抗体的功能以及线虫抗原的影响。

• 批准号: 06670266
• 负责人: UCHIKAWA Ryuichi
• 金额: $ 1.34万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670266/
• 关键词: IgE; RAT; Nippostrongylus brasiliensis; T CELL; CYTOKINE; TH2 CELL; ES ANTIGEN; PARASITE; rat; nematode; IFN-g; 腸間膜リンパ節

1. The percentages CD4^+ and CD8^+ T cell in the mesenteric lymph node (MLN) were examined by a Flow cytometory. MLN has been reported a main site for IgE production. The increase of CD4^+ and the decrease of CD8^+ T cell proportions were observed in MLN from Nippostrongylus brasiliensis infected rats. The percentage of CD8^+ T cell in MLN from a low-IgE-responder strain, Fischer (F)-344, was higher than that from Brown Norway (BN) rat, a high-IgE-responder, before and after the nematode infection.2. MLN cells from F-344 produced more interferon (IFN)-gamma than those from BN rat. The IFN-gamma production was suppressed by the nematode infection. The difference in IFN-gamma production by MLN was correlated with the relative proportion of CD8^+ T cell in MLN.We confirmed that IFN-gamma was mainly produced by T cell also in rats.3. mRNA expressions of IL-3, IL-4 and IL-5 increased after the nematode infection in both rat strains, while was no clear changed in the expression of IL-2, IL-10 and IFN-gamma, indicating that Th2 cells were induced by the nematode infection in rats. The expressions of IL-3, IL-4 and IL-5 in BN rat were higher than those in F-344 before and after the infection.4. ES antigen and adult worm derived purified Cysteine protease (Cys P) suppressed Con A driven IFN-gamma production by MLN cells in dose dependent manner without cytotoxic activity of the antigens.

1.用流式细胞仪检测肠系膜淋巴结（MLN）中CD 4 ^+和CD 8 ^+ T细胞的百分率。已报道MLN是IgE产生的主要场所。感染巴西日本圆线虫大鼠MLN中CD 4 ^+ T细胞比例升高，CD 8 ^+ T细胞比例降低。在线虫感染前后，低IgE反应株Fischer（F）-344的MLN中CD 8 ^+ T细胞的百分比高于高IgE反应株Brown Norway（BN）大鼠的MLN中CD 8 ^+ T细胞的百分比。MLN细胞从F-344产生更多的干扰素（IFN）-γ比BN大鼠。IFN-γ的产生受到线虫感染的抑制。MLN产生IFN-γ的差异与MLN中CD 8 ^+ T细胞的相对比例有关，我们证实大鼠的IFN-γ也主要由T细胞产生.两品系大鼠感染线虫后IL-3、IL-4和IL-5的mRNA表达均升高，而IL-2、IL-10和IFN-γ的表达无明显变化，提示线虫感染诱导了大鼠Th 2细胞的产生。感染前后BN大鼠IL-3、IL-4、IL-5的表达均高于F-344大鼠. ES抗原和成虫来源的纯化半胱氨酸蛋白酶（Cys P）以剂量依赖性方式抑制MLN细胞的Con A驱动的IFN-γ产生，而没有抗原的细胞毒性活性。

项目成果

UCHIKAWA,R: "IgE anti body production is associated with Auppessed in terfeton-8 leuels in mesen teric lympl modies of rats infected with the ramatade Nippstrongylus brasilidulsis." Immunolgy. 82. 427-432 (1994)

UCHIKAWA,R：“IgE 抗体的产生与感染巴西圆线虫的大鼠肠系膜淋巴模式中的 terfeton-8 leuels 中的 Auppessed 有关。”

MATSUDA,S: "Cytokine mRNA expression plofiles in rats infected with the intestiral nematode Nippostrongylus brasiliensis" Infection and Immunity. 63. 4653-4660 (1995)

MATSUDA,S：“感染肠线虫巴西圆线虫的大鼠中的细胞因子 mRNA 表达谱”感染和免疫。

UCHIKAWA,R. ,Yamada,M. ,MATSUDA,S. ,S: "Dissociation of early and late pro tectiue immunity to the nematode xippostiongylus brasiliensis in Brown Nanway and Fishev-344 rats" Parasitology. 112(印刷中). (1996)

UCHIKAWA, R.、Yamada, M.、MATSUDA, S.、S：“Brown Nanway 和 Fishev-344 大鼠中对巴西线虫的早期和晚期保护性免疫的分离”，《寄生虫学》112（出版中）。 ）

Uchikawa, R., Yamada, M., and Arizono, N.: "IgE antibody production is associated with suppressed interferon-gamma levels in mesenteric lymph nodes of rats infected with the nematode Nippostrongylus brasiliensis." Immunology. 82(3). 427-432 (1994)

Uchikawa, R.、Yamada, M. 和 Arizono, N.：“IgE 抗体的产生与感染巴西圆线虫的大鼠肠系膜淋巴结中干扰素 γ 水平的抑制有关。”

UCHIKAWA,R: "Dissociation of early and late protective immunity to the nematode Nippostrongylus brasiliensis in Brown Norway and Fischer-344 rats" Parasitology. 112(印刷中). (1996)

UCHIKAWA, R：“棕色挪威和 Fischer-344 大鼠中对巴西圆线虫的早期和晚期保护性免疫的分离”《寄生虫学》112（出版中）。