Analysis of HIV-1 Specific Cytotxic T Lymphocyte: Recognition for Variant HIV-1 epitopes

HIV-1 特异性细胞毒性 T 淋巴细胞分析：HIV-1 变异表位的识别

• 批准号: 10670287
• 负责人: TOMIYAMA Hiroko
• 金额: $ 2.05万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670287/
• 关键词: HIV-1; CTL; HLA-class I; EPITOPE; TETRAMER; CTL; CTLエピトープ; HLA-B35; HLA-B51; エスケープミュータント

In this investigation we can get the result as follows.1. We could identified several new HIV-1 CTL epitope presented by HLA-A11, -A24, -B51 molecules which are common HLA-class I alleles in Japanese.2. To quantify HIV-1 specific CD8+ T cells in PBMC from chronically HIV-1 infected individuals, HLA-BィイD1*ィエD13501 tetrameric complexes for six HIV-1 CTL epitopes were generated. These HLA-BィイD1*ィエD13501 tetramers effectively identified HIV-1 specific CD8+ T cells in HIV-1 infected individuals with HLA-B35. CD8+ T cells specific for multiple HLA-BィイD1*ィエD13501-restricted epitopes were detected in these individuals.3. Tetramer positive CD8+ T cells were markedly reduced after suppression of HIV-1 replication by highly active antiretroviral therapy.4. The recognition of HIV-1 specific CD8+ T cells for HIV-1 variant epitopes in HIV-1 infected individuals are now under investigation

在本次调查中，我们可以得到如下结果：1.结果1.我们发现了几个新的HIV-1CTL表位，它们是日本常见的人类白细胞抗原I类等位基因，由人类白细胞抗原A11、-A24、-B51分子提出。为定量检测HIV-1慢性感染者外周血单核细胞中HIV-1特异性CD8+T细胞的数量，构建了针对6个HIV-1CTL表位的HLABィイD1*ィエD13501四聚体。这些HLABィイD1*ィエD13501四聚体有效地识别了HIVB35感染者中HIV1特异性CD8+T细胞。在这些个体中检测到多个ィイ-B CD1*ィエD13501限制性表位的CD8+T细胞。经高效抗逆转录病毒治疗抑制HIV-1复制后，四聚体阳性CD8+T细胞明显减少。HIV-1感染者体内HIV-1特异性CD8+T细胞对HIV-1变异表位的识别作用正在研究中

项目成果

Hiroko Tomiyama: "Identification of Multiple HIV-1 CTL Epitopes Presented by HLA-B^*5101 Molecules"Human Immunology. 60. 177-186 (1999)

Hiroko Tomiyama：“由 HLA-B^*5101 分子呈现的多个 HIV-1 CTL 表位的鉴定”人类免疫学。

Katsuhiko Fukada, Yoshitomo Chujoh, Hiroko Tomiyama, Kiyoshi Miwa, Yutaro Kaneko, Shinichi Oka and Masafumi Takiguchi: "HLA-AィイD1*ィエD11101-restricted cytotoxic T lymphocyte recognition of HIV-1 Pol protein."AIDS. 13. 1413-114 (1999)

Katsuhiko Fukada、Yoshitomo Chujoh、Hiroko Tomiyama、Kiyoshi Miwa、Yutaro Kaneko、Shinichi Oka 和 Masafumi Takiguchi：“HLA-A D1*D11101 限制性细胞毒性 T 淋巴细胞对 HIV-1 Pol 蛋白的识别。”AIDS 13. 1413-114（ 1999）

Ai Kawana: "Accumulation of Specific Amino Acid Substitutions in HLA-B35-Restricted Human Immunodeficiency Virus Type I Cytotoxic T Lymphocyte Epitopes"AIDS Research and Human Retroviruses. 15. 1099-1107 (1999)

Ai Kawana：“HLA-B35 限制性人类免疫缺陷病毒 I 型细胞毒性 T 淋巴细胞表位中特定氨基酸取代的积累”艾滋病研究和人类逆转录病毒。

Masafumi Takiguchi: "Analysis of three HLA-A^*3303 bindin peptide anchors using an HLA-A^*3303 stabilization assay"Tissue Antigens. 55 ( in press ). (2000)

Masafumi Takiguchi：“使用 HLA-A^*3303 稳定测定法分析三个 HLA-A^*3303 结合肽锚”组织抗原。

Hiroko Tomiyama: "Expansion of HIV-1-Specific CD28-45RA-CD8^+ T cells in chromically HIV-1 infected individuals"AIDS. ( in press ). (2000)

Hiroko Tomiyama：“HIV-1 特异性 CD28-45RA-CD8^ T 细胞在慢性 HIV-1 感染个体中的扩增”艾滋病。