Analysis of HIV-1 Specific Cytotxic T Lymphocyte: Recognition for Variant HIV-1 epitopes

在这次调查中，我们可以得到结果，如以下：我们可以通过HLA-A11、-A24和-B51鉴定出几种新的HIV-1 CTL外延，这些分子在日本是常见的HLA类我推荐的。To quantify HIV-1 specific CD8+ T cells in PBMC from？ally HIV-1 infected individuals， HLA-BイD1* イエD13501 tetrameric complexes for six HIV-1 CTL epitopes were generated。These HLA-B病毒D1* 病毒D13501 tetramers effectively identified HIV-1 specific CD8+ T cells in HIV-1 infected individuals with HLA-B35。CD8+ T cells specific for multiple HLA-B-D1*-D13501-res？ed epitopes were detected in these individuals.3。Tetramer positive CD8+ T cells were markedly reduced after suppression of HIV-1 replication by highly active antiretroviral therapy.4. HIV-1感染个体中HIV-1特定CD 8 + T细胞的识别结果目前仍在调查中

In this investigation we can get the result as follows.1. We could identified several new HIV-1 CTL epitope presented by HLA-A11, -A24, -B51 molecules which are common HLA-class I alleles in Japanese.2. To quantify HIV-1 specific CD8+ T cells in PBMC from chronically HIV-1 infected individuals, HLA-BィイD1*ィエD13501 tetrameric complexes for six HIV-1 CTL epitopes were generated. These HLA-BィイD1*ィエD13501 tetramers effectively identified HIV-1 specific CD8+ T cells in HIV-1 infected individuals with HLA-B35. CD8+ T cells specific for multiple HLA-BィイD1*ィエD13501-restricted epitopes were detected in these individuals.3. Tetramer positive CD8+ T cells were markedly reduced after suppression of HIV-1 replication by highly active antiretroviral therapy.4. The recognition of HIV-1 specific CD8+ T cells for HIV-1 variant epitopes in HIV-1 infected individuals are now under investigation