HLA CLASS I MOLECULES AND THE CTL RESPONSE TO HIV

HLA I 类分子和 CTL 对 HIV 的反应

• 批准号: 2671992
• 负责人: JEFFREY Allen FRELINGER
• 金额: $ 26.91万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2671992
• 关键词: MHC class I antigen; X ray crystallography; antigen presentation; cellular immunity; cytotoxic T lymphocyte; gene mutation; genetically modified animals; human immunodeficiency virus 1; human tissue; laboratory mouse; microorganism immunology; molecular cloning; tissue /cell culture; transfection; virus antigen

DESCRIPTION: The objective of this application is to better understand the role of cytotoxic T lymphocytes in the recognition and destruction of HIV-infected cells. The investigator has particularly focused on the role of major histocompatibility complex encoded class I molecules and their interactions with peptides. He has used the technologies of molecular biology and cellular immunology to devise strategies for improving the peptide epitopes themselves and used a variety of delivery systems in an attempt to improve delivery to cells and to experimental animals. Of particular novelty is the use of transgenic mice to produce cytotoxic responses to HIV encoded proteins, but which use the human MHC peptide binding molecules to better mimic human antigen presentation. These experiments are designed to allow a better understanding and are pointed towards more rational vaccine design and delivery systems.

描述：本申请的目的是为了更好地了解 细胞毒性T淋巴细胞在识别和破坏 HIV感染细胞 调查员特别关注的是 主要组织相容性复合体编码的I类分子及其 与肽的相互作用。 他利用分子技术 生物学和细胞免疫学来设计策略， 肽表位本身，并使用各种递送系统， 试图改善对细胞和实验动物的递送。 的 特别新奇的是使用转基因小鼠来产生细胞毒性 对HIV编码的蛋白质的反应，但其使用人MHC肽 结合分子以更好地模拟人类抗原呈递。 这些 实验的目的是为了更好地理解，并指出 更合理的疫苗设计和输送系统。