HLA CLASS I MOLECULES AND THE CTL RESPONSE TO HIV

HLA I 类分子和 CTL 对 HIV 的反应

• 批准号: 2064921
• 负责人: JEFFREY Allen FRELINGER
• 金额: $ 19.87万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1996-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2064921
• 关键词: AIDS; Escherichia coli; HIV infections; MHC class I antigen; T cell receptor; cellular immunity; chemical binding; cytotoxic T lymphocyte; gene mutation; genetic library; genetic manipulation; human immunodeficiency virus 1; human subject; leukocyte activation /transformation; protein purification; site directed mutagenesis; tissue /cell culture; virus protein

The HLA class I molecules are crucial in the recognition by cytolytic T lymphocytes (CTL) of their targets. In virus infected cells, fragments of viral peptides bind to class I molecules and this class I/peptide complex is recognized by CTLs. T cells are important in the clearance of nearly all virus infections. In the experiments proposed here we will examine the binding of HIV encoded peptides to MHC class I molecules, and the relationship between binding and CTL recognition. In addition, we will undertake a detailed analysis of the interaction between HIV peptides and class I products using a combination of peptide synthesis, and saturation mutagenesis of the HLA genes. These experiments will provide important information on the binding of HIV peptides of HLA and lay the groundwork for the development of synthetic vaccines which can take into account the variability of HLA.

HLA I 类分子对于溶细胞 T 的识别至关重要 其目标的淋巴细胞（CTL）。在病毒感染的细胞中， 病毒肽与 I 类分子和 I 类/肽复合物结合 被 CTL 认可。 T 细胞对于几乎所有的清除都很重要 病毒感染。在这里提出的实验中，我们将检查 HIV编码肽与MHC I类分子的结合，以及 结合和CTL识别之间的关系。此外，我们将 对 HIV 肽和 HIV 肽之间的相互作用进行详细分析 使用肽合成和饱和相结合的 I 类产品 HLA 基因的突变。这些实验将提供重要的 HIV 肽与 HLA 结合的信息并奠定基础 开发合成疫苗，可以考虑到 HLA 的变异性。