Mechanism of toxin synthesis using cultured poisonous mushroom

人工培养毒蘑菇合成毒素的机制

• 批准号: 10670380
• 负责人: GONMORI Kunio
• 金额: $ 2.3万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670380/
• 关键词: Mushroom poisoning; Cultivation; Magic mushroom; Growth factor; Analysis; HPLC; 毒きのこ; 幻覚きのこ; 毒成分

Several kind of mushrooms has strong toxicity to kill people. However, there have been only few reports of toxicological analysis on poisonous mushroom in forensic field. This study aimed to cultivate some poisonous mushrooms for the purpose of investigation and research in forensic toxicology. In our experiment the following mushrooms were cultivated, Pleurotus ostreatus, Lentinus edodes, Psilocybe cubensis, Amanita virosa, Lampteromyces japonicus and Gymnopilus spectabilis. Eventually the cultivation of Lyophyllum and Psilocybe cubensis resulted well-grown mushrooms. In the case of the Psilocybe cubensis, psilocybin and psilocin are the main hallucinogenic components. We observed the effect of storage on the concentration of these two hallucinogens. From harvesting the Psilocybe cubensis the freezing method provided the most valuable results to store mushrooms. A sample from a poisonous case suggested magic mushroom ingestion was analyzed. The mushroom was described as Psilocybe subcubensis. Recently the interest of the magic mushroom poisoning appears to be growing around the world. This research attempts to promote the investigation protocol for magic mushroom's cases.

几种蘑菇具有很强的致死毒性。然而，在法医学领域对有毒蘑菇进行毒理分析的报道却很少。本研究旨在培养出一些有毒的蘑菇，为法医毒理学的调查和研究做准备。本试验以平菇、香菇、平菇、鹅膏菌、日本蘑菇、明盖平菇为试材，研究了平菇、香菇、平菇、鹅膏菌、平菇、白花菇、平菇、最终，通过栽培番茄和南美裸盖蘑菇，获得了长势良好的蘑菇。在裸盖裸盖酵母的情况下，裸盖菇素和裸盖菇素是主要的致幻成分。我们观察了储存对这两种致幻剂浓度的影响。从采收的小球藻来看，冷冻法为蘑菇的贮藏提供了最有价值的结果。对一起中毒病例的样本进行了分析，表明他们摄入了魔力蘑菇。这种蘑菇被描述为亚立方体拟裸盖菌属。最近，神奇蘑菇中毒事件似乎在世界各地引起了越来越大的兴趣。本研究试图推广魔菇案件的侦查方案。

项目成果

権守邦夫,吉岡尚文: "当教室で経験したきのこ中毒検査事例"秋田県救命救急研究会誌. 5. 74-78 (2001)

Kunio Gomori、Naofumi Yoshioka：“在我们的课堂上经历的蘑菇中毒测试案例”秋田县重症监护研究会杂志5. 74-78（2001）。

KUNIO GONMORI, NAOFUMI YOSHIOKA: "Poisoning Cases by Mushroom"Journal of Akia Emergency Medicine. 5. 74-78 (2001)

KUNIO GONMORI、NAOFUMI YOSHIOKA：“蘑菇中毒病例”Akia 急诊医学杂志。