Development of the Analytical Methods of the Mushroom Toxin

点击翻译按钮获取中文摘要

Rhodophyllus rhodopolius (Fr.) Quel. has been known in Japan as a poisonous mushroom. According to the annual statistical data from Japanese Ministry of Health and Welfare, R. rhodopolius came in the second to cause poisoning by mushrooms. The Rhodophyllus species are generally harmless, and the toxic R. rhodopolius is often mistaken by amateur mushroom hunters for the edible mushroom Rhodophyllus crassipes, since both species are morphologically very similar. The development of the sensitive and simple methods for routinely discriminating the mushroom between edible and poisonous species are required.In this report the effective methods by means of coloring reagent, spectrophotometry and high-performance liquid chromatography with electro-chemical detector are described. And also the affect of mushroom toxin for the biogenic amines in rat in vitro and in vivo experiments are studied. As the results, the coloring reagent cannot discriminate the mushrooms between edible and poisonous species. On the other hand the use of high-performance liquid chromatography with electro- chemical detector might be practical for distinguishing the R. rhodopolius from R. crassipes. Increased of 5-HT and dopamine content in rat brain were observed by in vivo experiments. But further experiments must be required to find out the mechanism.