Analysis of the function of macrophages from mice infected with MADIDS.

IL-18， a产品活性macrophages and Kupffer cells一项专利pleiotropic cytokine. IL-18 induces IFN-γproduction by lymphocytes, such as T cells，natural killer (NK) cellsparticularly in a synergistic manner with IL-12. IL-18 augments NK cell activity through theactivation of constitutively express IL-18 receptor on NK cells. In addition，IL-18 upregulates Fas ligand-mediated cytotoxic activity of cloned Th1 cells and NK cells. IL-18also directly activates CD8 - D1+ D1T cells，which play a central role in viral clearance. Additionally，IL-18和IL-12是accumulated after infection with microbes or microbe productsallowing us to propose the possibility that IL-18 along with IL-12 participates in microbe clearanceand furthermore exogenous IL-18 might enhance host defense. C57BL/6 mice inolated with themixture of murine leukemia viruses (MuL Vs) called LP-BM5 MuLV develop an immunodeficiency syndromewhich in many ways resembles that seen in humans infected with human immunodecifiency virus type-1The function of macrophages and NK cells in HIV infection play a key role developing Thediseases. Thus，我们examined the function in mouse infected with LP-BM5 MuLV，murine AIDS. We也曾examined the contribution of IL-18 in developing of MAIDS. the results首先，我们revealed serum IL-18 was increased during the course of MAIDS，~ 5μg/ml at 16 weeks after infection . Second，macrophages from MAIDS which infected 8 weeks before produced IL-18 in response to LPSwhile those from normal control C57BL/6 mice did not. Third, the expression of B7.2as an activated cell surface marker on MAIDS macrophages was增强. Family，NK activity was augmented 8 weeks after infection with MAIDS

IL-18, a product of activated macrophages and Kupffer cells, is a potent pleiotropic cytokine. IL-18 induces IFN-γproduction by lymphocytes, such as T cells, and natural killer (NK) cells, particularly in a synergistic manner with IL-12. IL-18 augments NK cell activity through the activation of constitutively express IL-18 receptor on NK cells. In addition, IL-18 upregulates Fas ligand-mediated cytotoxic activity of cloned Th1 cells and NK cells. IL-18 also directly activates CD8ィイD1+ィエD1T cells, which play a central role in viral clearance. Additionally, IL-18 as well as IL-12 is accumulated after infection with microbes or microbe products, allowing us to propose the possibility that IL-18 along with IL-12 participates in microbe clearance and furthermore exogenous IL-18 might enhance host defense. C57BL/6 mice inoculated with the mixture of murine leukemia viruses (MuL Vs) called LP-BM5 MuLV develop an immunodeficiency syndrome which in many ways resembles that seen in humans infected with human immunodecifiency virus type-1 (HIV-1). The function of macrophages and NK cells in HIV infection play a key role developing the diseases. Thus, we examined the function in mouse infected with LP-BM5 MuLV, murine AIDS (MAIDS). We also examined the contribution of IL-18 in developing of MAIDS. The results are following. First, we revealed that serum IL-18 was increased during the course of MAIDS, 〜5μg/ml at 16 weeks after infection . Second, macrophages from MAIDS which infected 8 weeks before produced IL-18 in response to LPS, while those from normal control C57BL/6 mice did not. Third, the expression of B7.2, as an activated cell surface marker on MAIDS macrophages was enhanced. Family, NK activity was augmented 8 weeks after infection with MAIDS.