Research on the structure and functions of human FTZ-F1β gene

人FTZ-F1β基因结构与功能研究

• 批准号: 10671039
• 负责人: YANASE Toshihiko
• 金额: $ 2.5万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671039/
• 关键词: FTZ-F1β; Ad4BP; FTZ-F_1β; mLRH-1; FTZ-F1

FTZ-F1β had been implicated to be a counterpart of Ad4BP and mainly expressed in liver and pancreas. We elucidated the structure of the human FTZ-F1β gene. We determined the exonic sequences including the exon/intron baundaries and the sequences at the 5'-flanking region. The gene is at least 50 Kb long and is split into 8 exons including a non-coding exon 1. The overall structural organization of human FTZ-F1β gene was very similar to that of human Ad4BP/SF-1 gene, suggesting that both genes are derived from common ancestor gene. In addition, a preliminary study of transcriptional regulation of human FTZ-F1β gene in Hep G2 cells using a series of deletion analysies of the 5'-flanking region of human FTZ-F1β gene revealed a upstream region of -1743 to -1040 bp from the transcriptional statsite to be essential for the basal transcription of the human FTZ-F1β gene in Hep G2 cells.

FTZ-F1β被认为是Ad4BP的对应物，主要在肝脏和胰腺中表达。我们确定了人FTZ-F1β基因的结构。我们确定了外显子序列，包括外显子/内含子边界和5'侧区域的序列。该基因至少50 Kb长，分为8个外显子，包括一个非编码外显子1。人类FTZ-F1β基因的整体结构组织与人类Ad4BP/SF-1基因非常相似，表明两者均来源于共同祖先基因。此外，通过对人FTZ-F1β基因5'侧区域的一系列缺失分析，对人FTZ-F1β基因在Hep G2细胞中的转录调控进行了初步研究，发现转录位点上游-1743至-1040 bp的区域对人FTZ-F1β基因在Hep G2细胞中的基础转录至关重要。

项目成果

Nawata, H., Yanase, T.et al.: "Human Ad4BP/SF-1 and its related nuclear receptor"J Steroid Biochem Molec. 69. 323-328 (1999)

Nawata, H., Yanase, T.等人：“人类 Ad4BP/SF-1 及其相关核受体”J Steroid Biochem Molec。

Oba K,Yanase T et al.: "Transcriptional regulation of human FTZ-F1 gene encoding Ad4BP/SF-1"J Biochem. 128. 517-528 (2000)

Oba K，Yanase T 等人：“编码 Ad4BP/SF-1 的人 FTZ-F1 基因的转录调控”J Biochem。

Nawata H, Yanase T et al.: "Human Ad4BP/SF-1 and its velated nuclear receptor" J. Steroid Biochiem Molec. 印刷中. (1999)

Nawata H、Yanase T 等人：“人类 Ad4BP/SF-1 及其相关核受体”J. Steroid Biochiem Molec 出版（1999 年）。

Hirase N,Yanase T, et al.: "Thiazolidinedione suppresses the expression of erythroid phenotype in erythroleukemia cell line K562."Leukemia Research. 24. 393-400 (2000)

Hirase N、Yanase T 等人：“噻唑烷二酮抑制红白血病细胞系 K562 中红系表型的表达。”白血病研究。

Oba K, Yanase T et al.: "Transcriptional regulation of hurman FTZ-F1 gene encoding Ad4BP/SF-1"J Biochem. 128. 517-528 (2000)

Oba K、Yanase T 等人：“编码 Ad4BP/SF-1 的 hurman FTZ-F1 基因的转录调控”J Biochem。