Research on the structure and functions of human FTZ-F1β gene

FTZ-F1β被认为是Ad4BP的对应物，主要在肝脏和胰腺中表达。我们确定了人FTZ-F1β基因的结构。我们确定了外显子序列，包括外显子/内含子边界和5'侧区域的序列。该基因至少50 Kb长，分为8个外显子，包括一个非编码外显子1。人类FTZ-F1β基因的整体结构组织与人类Ad4BP/SF-1基因非常相似，表明两者均来源于共同祖先基因。此外，通过对人FTZ-F1β基因5'侧区域的一系列缺失分析，对人FTZ-F1β基因在Hep G2细胞中的转录调控进行了初步研究，发现转录位点上游-1743至-1040 bp的区域对人FTZ-F1β基因在Hep G2细胞中的基础转录至关重要。

FTZ-F1β had been implicated to be a counterpart of Ad4BP and mainly expressed in liver and pancreas. We elucidated the structure of the human FTZ-F1β gene. We determined the exonic sequences including the exon/intron baundaries and the sequences at the 5'-flanking region. The gene is at least 50 Kb long and is split into 8 exons including a non-coding exon 1. The overall structural organization of human FTZ-F1β gene was very similar to that of human Ad4BP/SF-1 gene, suggesting that both genes are derived from common ancestor gene. In addition, a preliminary study of transcriptional regulation of human FTZ-F1β gene in Hep G2 cells using a series of deletion analysies of the 5'-flanking region of human FTZ-F1β gene revealed a upstream region of -1743 to -1040 bp from the transcriptional statsite to be essential for the basal transcription of the human FTZ-F1β gene in Hep G2 cells.