AN EXPERIMENTAL STUDY OF MACROENCAPSULATED ARTIFICIAL PANCREAS USING HOLLOW FIBER DERIVED FROM POLYSULFONE MEMBRANE

采用Liberase法分离大鼠胰岛，观察胰岛的产量和功能。采用内径0.5mm、外径0.8mm、长60 cm的聚砜中空纤维作为微囊材料，采用Field等新开发的梯度分离技术，其中将白蛋白包被（35%BSA）的大鼠胰岛分离在右旋糖酐中。胰岛产量为875±746个/胰腺。通过灌流研究胰岛的活力，其中人工胰腺在含有60 mg/dl葡萄糖浓度的RPMI溶液中孵育30分钟，然后在300 mg/dl葡萄糖下孵育30分钟，最后在60 mg/dl葡萄糖下孵育30分钟。值得研究作为巨囊化膜的可能性。需要进一步调查。

The yield and function of rat pancreatic islets were investigated, using Liberase for isolation. We used polysulfone hollow fiber as macroencapsulation materials, which the inner diameter was 0.5 mm, the outer one was 0.8 mm, and the length was 60 cm.We used a gradient separation technique newly developed by Field et al., in which albumin-coated (35%BSA) rat islets were isolated in Dextran. The islets yield were 875±746 per pancreas. The viability of islets were investigated by perifusion which an artificial pancreas was incubated in RPMI solution containing a glucose concentration of 60mg/dl for 30 minutes followed by a 30 minute period of glucose at 300 mg/dl followed by a final period of glucose at 60 mg/dl.Macroencapsulated islets responded well to glucose stimulation.Polysulfone hollow fiber, which is biocompatible since deriving from dyalyzer, is worth examining the possibility as an membrane for macroencapsulation. Further investigation will be necessary.