Study on endocrine disruptors of dental materials using an amphibian system

通过酵母和细菌的筛选试验，如竞争性雌激素受体结合试验和含有这些化学物质的雌激素反应启动子的基因报告试验，逐渐明确了EDCs的风险评估。然而，还需要生物测定系统来阐明化合物对动物和人类的直接影响，因为环境化合物可能在体内或细胞中代谢转化为雌激素或非雌激素物质。因此，有必要进行雌激素和甲状腺激素活性的筛选试验，并利用两栖动物系统开发了新的系统。利用该系统对口腔材料的雌激素和甲状腺激素活性进行了评价。结果表明，部分牙科材料显示雌激素和甲状腺激素的活性。

Risk assessment of EDCs have gradually been clarified by the screening test that used a yeast and bacteria such as competitive estrogen receptor-binding assay and reporter assay of genes containing estrogen-responsive promoters about these chemical substance. However, bioassay systems are also needed to elucidate direct effects of the compounds on animals and humans because environmental compounds may be metabolically transformed into estrogenic or non-estrogenic substances in the body or cells. Therefore, the screening test of estrogen and thyroid hormone activities is needed and we developed the new system using an amphibian system. By this system we evaluated the estrogen and thyroid hormone activities of dental materials. As the result, some of dental materials indicated the estrogen and thyroid hormone activities.