Vascular endothelial growth factor-C mRNA expression on lymphatic metastasis of thyroid papillary carcinoma

血管内皮生长因子-C mRNA在甲状腺乳头状癌淋巴转移中的表达

• 批准号: 10671139
• 负责人: OBARA Takao
• 金额: $ 2.37万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671139/
• 关键词: vascular endothelial growth factor; papillary thyroid carcinoma; follicular thyroid carcinoma; metastasis; 甲状腺癌; 血管内皮細胞増殖増殖因子; 発癌機序

Background :Vascular endothelial growth factor-C (VEGF-C) is known to be related to development of lymphatic vessels. Papillary thyroid carcinoma characteristically metastasizes to regional lymph nodes, whereas follicular thyroid carcinoma commonly spreads hematogenously. The present study was designed to determine whether expression of the VEGF-C gene is related to the different metastatic features of these 2 types of thyroid carcinoma.Methods :Tyroid carcinoma specimens were obtained from15 patients with papillary carcinoma and 4 patients with follicular carcinoma of the thyroid. VEGF-C gene expression was exmined by Northern blotting and in situ hybridization. Immunohistochemistry was performed to localize the deposition of VEGF-C protein.Results :The ratios of VEGF-C gene expression determined by Northern blot analysis were significantly higher in papillary than in follicular carcinoma. Nonmalignant thyroid tissue from patients with papillary carcinoma also expressed higher levels of VEGF-C than tissue from patients with follicular carcinoma. Expression of the VEGF-C gene was observed by in situ hybridization in cells of papillary thyroid carcinoma but not in those of follicular carcinoma. Positive staining with antibody against VEGF-C was detected in papillary cancer cells.Conclusions :Concurent overexpression of the VEGF-C gene by both tumor cells and the surrounding tissue may be related to the prevalence of intrathyroidal spread through lymphatics and regional lymph node metastasis in patients with papillary carcinoma.

背景：血管内皮生长因子-C(VEGF-C)与淋巴管的发育有关。甲状腺乳头状癌的特点是转移到区域淋巴结，而滤泡性甲状腺癌通常通过血液扩散。本研究旨在探讨血管内皮生长因子-C基因的表达与两种类型甲状腺癌不同转移特征的关系。方法：收集15例甲状腺乳头状癌和4例滤泡性甲状腺癌标本。Northern印迹和原位杂交检测血管内皮生长因子-C基因的表达。结果：Northern印迹法检测乳头状癌组织中VEGF-C基因的表达比例明显高于滤泡性癌组织。乳头状癌患者的非恶性甲状腺组织表达的VEGF-C水平也高于滤泡性癌患者的组织。原位杂交结果显示，VEGF-C基因在甲状腺乳头状癌中有表达，而在滤泡性癌中未见表达。结论：肿瘤细胞和癌旁组织中均有VEGF-C基因的持续过表达，这可能与乳头状癌患者甲状腺内淋巴管扩散和区域淋巴结转移有关。

项目成果

Peter Felmer: "Vascular endothelial growth factor-C gene expression in papillary and follicular thyroid carcinomas."SURGERY. 126(6). 1056-1062 (1999)

Peter Felmer：“甲状腺乳头状癌和滤泡状癌中的血管内皮生长因子-C 基因表达。”外科。

Peter Fellmer: "Vascular endothelial growth factor-C gene expression in papillary and follicular thyroid carcinomas."SURGERY. 126. 1056-1062 (1999)

Peter Fellmer：“甲状腺乳头状癌和滤泡状癌中的血管内皮生长因子-C 基因表达。”外科。

Peter Fellmet: "Vascular endothelial growth factor-C gene expression in papillary and follicular theroid carcinoma"Surgery. 126(6). 1056-1062 (1999)

Peter Fellmet：“血管内皮生长因子-C 基因在乳头状和滤泡性乳头状癌中的表达”外科。