Tubulin plays important role in morphogenesis of cellular and structural atypia in papillary thyroid carcinoma tissue

本研究的目的是阐明甲状腺癌中细胞和结构异常形态发生的分子背景。我们建立了甲状腺乳头状癌细胞培养体系，在此体系中，培养的甲状腺乳头状癌细胞人工形成核沟（NV）和核内包涵体（PNI）。使用该培养系统，我们研究了NV/PNI细胞中α/β-和γ-微管蛋白、核膜蛋白和细胞骨架的表达。在NV附近的细胞质和PNI中检测到γ-微管蛋白。此外，我们还研究了手术切除的甲状腺癌组织中γ-微管蛋白和核膜蛋白的表达。癌组织中γ-微管蛋白分布和核膜蛋白的异常表达。结果表明，非中心体微管组织中心和核膜蛋白在培养细胞NV/PNI的形成和人癌组织中滤泡和乳头状结构的形态发生中起着重要作用。

Aim of the study was to clarify a molecular background of morphogenesis of cellular and structural atypia in thyroid cancer. We established culture system, in which cultured papillary thyroid carcinoma cells artificially formed nuclear grooves (NV) and pseudonuclear inclusion (PNI). Using the culture system, we investigated expression of alpha/beta- and gamma-tubulins, nuclear membrane proteins and cytoskeletons in the cells with NV/PNI. Gamma-tubulins were detected in cytoplasm near NV and in PNI. Also, we studied expression of gamma-tubulin and nuclear membrane proteins in surgically resected human thyroid cancer tissue. Aberrant expression of gamma-tubulin distribution and nuclear membrane proteins was found in the cancer tissue. From the results, we concluded non-centrosomal microtubule organizing center and nuclear membrane proteins played a central role in NV/PNI formation in culture cells and morphogenesis of follicular and papillary structure in human cancer tissue.