Genetic alterations in colorectal cancer and dysplasia (DS) in patients with ulcerative colitis (UC)

溃疡性结肠炎（UC）患者结直肠癌和不典型增生（DS）的基因改变

• 批准号: 10671161
• 负责人: WATANABE Toshiakil
• 金额: $ 1.54万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671161/
• 关键词: Ulcerative colitis; Cancer; Dysplasia; p53; adenoma; Microsatellite instability; P53

Genetic alterations in colorectal cancer and dysplasia (DS) in patients with ulcerative colitis (UC) were examined. Losses of heterozygosity (LOH) in chromosome 5q, 17p, 8p, 18q, 22p and microsatellite instability (MSI) were examined. LOH was observed in 36%(4/11)(5q), 67%(8/12)(17p), 59%(10/17)(8p), 0%(0/3)(18q) and 0%(0/3)(22p), which indicated the importance of the p53 gene in the development of colorectal tumors in UC. No lesions showed MSI. In an immunohistochemical analysis, the over expression of p53 was observed in 89% of invasive cancers, 70% of high-grade dysplasia, 57% of low-grade dysplasia and 0% of adenoma. This showed the effectiveness of immunohistochemical analysis for different diagnosis of adenoma and dysplasia in a clinical setting.

研究了溃疡性结肠炎（UC）患者结直肠癌和发育不良（DS）的基因改变。检测了5q、17p、8p、18q、22p染色体杂合性损失（LOH）和微卫星不稳定性（MSI）。LOH分别为36%(4/11)（5q）、67%(8/12)（17p）、59%(10/17)（8p）、0%(0/3)（18q）和0%(0/3)(22p)，提示p53基因在UC结直肠肿瘤发生发展中的重要作用。未见MSI病变。在免疫组织化学分析中，89%的浸润性癌症、70%的高级别不典型增生、57%的低级别不典型增生和0%的腺瘤中观察到p53的过表达。这表明免疫组织化学分析在临床上对腺瘤和不典型增生的不同诊断的有效性。

项目成果

Watanabe T. Muto T.: "Recent advances in the treatment of rectal carcinoma"Critical Reviews in Oncology-Hematology. 32(1). 5-17 (1999)

Watanabe T. Muto T.：“直肠癌治疗的最新进展”肿瘤学-血液学批判性评论。

Matsuda K.Watanabe T.: "Ulcerative colitis patients with a family history of colorectal cancer should be subjected to close and careful surveillance"Japanese Journal of Clinical Oncology. 29(9). 448-451 (1999)

Matsuda K.Watanabe T.：“有结直肠癌家族史的溃疡性结肠炎患者应接受密切和仔细的监测”《日本临床肿瘤学杂志》。

Matsuda K., Masaki T., Ishii S., Yamashita H., Watanabe T., Nagawa H., Muto T., Hirata Y., Kimura K., Kojima S.: "Possible associations of rectal carcinoma with Schistosoma japonicum infection and membranous nephropathy : a case report with a review"Japan

Matsuda K.、Masaki T.、Ishii S.、Yamashita H.、Watanabe T.、Nakawa H.、Muto T.、Hirata Y.、Kimura K.、Kojima S.：“直肠癌与日本血吸虫感染的可能关联

Watanabe T. Muto T.: "Colorectal carcinogenesis based on molecular biology of early colorectal cancer, with special reference to nonpolypoid (superficial) lesions"World Journal of Surgery. 24(9). 1091-1097 (2000)

Watanabe T. Muto T.：“基于早期结直肠癌分子生物学的结直肠癌发生，特别是非息肉样（浅表）病变”《世界外科杂志》。

Watanabe T., Wu TT.: "Molecular predictors of survival after adjuvant chemotherapy for colon cancer"New England Journal of Medicine. 344(16). 1196-1206 (2001)

Watanabe T.，Wu TT.：“结肠癌辅助化疗后生存的分子预测因素”新英格兰医学杂志。