Establishment of highly metastatic models for human gastric cancer in nude mice and analysis of factors that regulate the metastatic modes.

人胃癌裸鼠高转移模型的建立及转移模式调控因素分析

基本信息

  • 批准号:
    10671204
  • 负责人:
  • 金额:
    $ 0.45万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 1999
  • 项目状态:
    已结题

项目摘要

The actual mechanisms that regulate various models of metastasis by human gastric cancer are still unclear, and there is a need to establish in vivo experimental models suitable for the investigation of hematogenous, lymphogenous and peritoneal metastases. For the purpose, we established highly liver-metastasizing line, designated AZH5G, lymph node-metastasizing line, AZL5G, and peritoneum-metastasizing line, AZ-P7a, which were derived from the same human gastric cancer cell line, AZ521, with low metastatic potential using in vivo stepwise selection in nude mice.All of three high-metastatic lines showed clearly increased motility and growth activity compared with the parental AZ521 cells, indicating that those properties are involved in the enhancement of metastatic potential regardless of metastatic modes. Concerning cell surface adhesion molecules, AZL5G expressed suggestive up-regulation of α2, α3, α5, α6 and αυβ3 integrins while AZH5G showed increased expression of α3 and α5 integrins and decreased expression of α6 integrin. The other adhesion molecules such as CD44 family, immunoglobulin superfamily or cadherin were not expressed in any cell lines. The adhesive activity of AZL5G cells to type-4 collagen and fibronectin was clearly increased than that of AZ521 cells, while contrast, the adhesive activity of AZ-P7a cells to these extracellular matrix proteins (ECMPs) was significantly decreased than that of AZ521 cells. These findings suggest that changes both in the expression of integrins and in adhesiveness to ECMPs may be involved in the regulation of metastatic mode in human gastric carcinomas.
调节人胃癌转移的各种模型的实际机制仍然不清楚,并且需要建立适合于调查血行、淋巴和腹膜转移的体内实验模型。为此,我们建立了高度肝转移细胞系,命名为AZH 5G,淋巴结转移细胞系,AZL 5G,和腹膜转移细胞系,AZ-P7 a,它们来源于相同的人胃癌细胞系AZ 521,在裸鼠体内逐步选择,所有三种高转移潜能的与亲本AZ 521细胞相比,转移系显示出明显增加的运动性和生长活性,表明这些性质与转移潜能的增强有关,而与转移模式无关。关于细胞表面粘附分子,AZL 5G表达α2、α3、α5、α6和α β3整合素,而AZH 5G显示α3和α5整合素表达增加,α6整合素表达减少。其他粘附分子如CD 44家族、免疫球蛋白超家族和钙粘蛋白在所有细胞系中均不表达。AZL 5G细胞对4型胶原和纤连蛋白的粘附活性明显高于AZ 521细胞,而AZ-P7 a细胞对这些细胞外基质蛋白的粘附活性明显低于AZ 521细胞。提示整合素和ECMPs的表达变化可能参与了胃癌转移方式的调节。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yamaguchi K.,Ura H.,Yasoshima T.,Shishido T.,Denno R.,Hirata K.: "Establishment and characterization of a human gastric carcinoma cell line that is highly metastatic to lymph node"Journal of Experimental and Clinical Cancer Reseach. (in press).
Yamaguchi K.、Ura H.、Yasoshima T.、Shishido T.、Denno R.、Hirata K.:“高度转移至淋巴结的人胃癌细胞系的建立和表征”实验与临床癌症研究杂志
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    0
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URA Hideki其他文献

URA Hideki的其他文献

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{{ truncateString('URA Hideki', 18)}}的其他基金

Changes of Thl/Th2 balance and serum cytokine levels in critically ill patients.
危重症患者Thl/Th2平衡及血清细胞因子水平的变化
  • 批准号:
    12671166
  • 财政年份:
    2000
  • 资助金额:
    $ 0.45万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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