Establishment of SCID mouse metastatic model of human malignant lymphoma and analysis of lectin binding properties on metastatic capacity

SCID小鼠人恶性淋巴瘤转移模型的建立及凝集素结合特性对转移能力的影响分析

• 批准号: 07670209
• 负责人: ABE Masafumi
• 金额: $ 1.34万
• 依托单位: Fukushima Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670209/
• 关键词: human B-cell lymphoma; SCID mouse; metastasis; lectin; carbohydrate; prognosis; 悪性リンパ腫; 培養株

1. Establishment of a metastatic SCID mouse model of human B-cell lymphoma lines :The human B-cell lymphoma lines (HBL-1, HBL-2, HBL-4, HBL-5, HBL-6, HBL-7, HBL-8, Daudi and Raji) were heterotransplanted subcutaneously into SCID mice. HBL-2, HBL-7 and HBL-8 cell lines showed a high spontaneous metastasis, whereas the other cell lines showed no or low-spontaneous metastasis. These high metastatic cell lines may be a useful tool to elucidate the metastatic mechanisms of human B-cell lymphoma.2. The relation between the binding sites for Glycine Max (SBA) lectin masked by sialylation and metastatic capacity in a SCID mouse model :A difference in cell surface carbohydrates between high-and no-or low-spontaneous metastatic human B-cell lymphoma lines were analyzed using lectin staining. The results showed that the masking of SBA lectin binding sites by sialic acid might be closely associated with metastatic capacity of human B-cell lymphoma lines in a SCID mouse model3. The relation between the lectin binding properties (VVA, ABA and PHA-L) and survival in 46 cases with human diffuse large B-cell lymphoma :The lectin binding properties on 46 cases with diffuse large B-cell lymphoma was analyzed in relation to survival. The lectin binding properties (VVA,ABA and PHA-L) were correlated with survival of patients with diffuse large B-cell lymphoma : The patients with lack or alterations of lectin binding sites of carbohydrates showed a worse prognosis in comparison to those without it.

1.人B细胞淋巴瘤细胞系SCID小鼠模型的建立：将人B细胞淋巴瘤细胞系HBL-1、HBL-2、HBL-4、HBL-5、HBL-6、HBL-7、HBL-8、Daudi和Raji移植到SCID小鼠皮下。HBL-2、HBL-7和HBL-8细胞系表现为高自发转移，而其他细胞系无自发转移或低自发转移。这些高转移细胞系可能是阐明人类B细胞淋巴瘤转移机制的有用工具。唾液酸化掩蔽的甘氨酸最大(SBA)凝集素结合位点与SCID小鼠模型转移能力的关系：用凝集素染色分析高转移性和无或低转移性B细胞淋巴瘤细胞表面碳水化合物的差异。结果表明，唾液酸对SBA凝集素结合部位的掩蔽可能与人B细胞淋巴瘤细胞系在SCID小鼠模型中的转移能力密切相关。46例弥漫性大B细胞淋巴瘤凝集素结合特性(VVA、ABA、PHA-L)与生存期的关系：分析46例弥漫性大B细胞淋巴瘤凝集素结合特性与生存期的关系。凝集素结合特性(VVA、ABA和PHA-L)与弥漫性大B细胞淋巴瘤患者的生存有关：糖类凝集素结合位点缺失或改变的患者预后较差。

项目成果

Masafumi,Abe: "Analysis of lectin binding properties on human Burkitt's lymphoma cell lines that show high spontaneous metastasis to distant organs in SCID mice:The binding sites for soybean agglutinin lectin masked by sialylation are closely associated w

Masafumi, Abe：“对人伯基特淋巴瘤细胞系的凝集素结合特性进行分析，这些细胞系在 SCID 小鼠中表现出高自发性远处器官转移：被唾液酸化掩盖的大豆凝集素凝集素的结合位点与

Masafumi, Abe: "Establishment and characterization of new human Burkitt's lymphoma cell lines (HBL-7 and HBL-8) that are highly metastatic in SCID mice : A metastatic SCID mouse model of human lymphoma lines" Pathology International. 46. 630-638 (1996)

Masafumi, Abe：“在 SCID 小鼠中高度转移的新型人类伯基特淋巴瘤细胞系（HBL-7 和 HBL-8）的建立和表征：人类淋巴瘤系的转移性 SCID 小鼠模型”病理学国际。

Masafumi, Abe: "Analysis of lectin binding properties on human Burkitt's lymphoma cell lines that show high spontaneous metastasis to distant organs in SCID mice : The binding sites for soybean agglutinin lectin masked by sialylation are closely associate

Masafumi, Abe：“对 SCID 小鼠中表现出高自发性远处器官转移的人伯基特淋巴瘤细胞系的凝集素结合特性进行分析：被唾液酸化掩盖的大豆凝集素凝集素的结合位点密切相关