Basic research of the multiple epileptic focal models

多种癫痫局灶模型的基础研究

• 批准号: 10671279
• 负责人: TANAKA Tasuya
• 金额: $ 1.98万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671279/
• 关键词: intractable epilepsy; kainic acid; animal model; multiple epileptic foci; callosal section; cortical epilepsy; experimental epilepsy; multiple cortical focus; Wistar rat; kaimic acid; experimental cortical focus; multiple focus; callosotomy

Basic approach to the multiple epileptic focal models were studied in cats and in rats. Cortical epileptogenic foci were induced by intracortical microinjections of kainic acid. In Experiment I, cats with a cortical focus, induced seizure did not propagate to the contralateral hemisphere after the callosal section. In animals with multiple cortical foci, after the callosal section, spontaneous seizures originated from each focus but the seizures localized to the ipsilateral hemisphere. In experiment II and III, the mode of seizure propagation was studied using EEG monitorings or autoradiographic study using 14C-deoxyglucose study. In animals without callosal section, three pathways were observed in the mood of seizure propagation ; 1) direct cortico-cortical pathway, 2) cotical focus to basal ganglia and thalamus, and 3) cortico cortical pathway via corpus callosum.In animals with callosal section, seizures did not become worse. However, autoradiogram demonstrated that seizures independently remained in both hemisphere even after the callosal section.The present study suggested that callosal section should be indicated after precise analysis of seizure type and seizure propagation in clinical application.

对猫和大鼠多灶性癫痫模型的基本方法进行了研究。皮质内微量注射海人酸致癫痫灶。在实验I中，大脑皮质病灶的猫，诱发癫痫发作后，并没有传播到对侧大脑半球后的穹窿。在有多个皮质病灶的动物中，自发性癫痫发作起源于每个病灶，但癫痫发作局限于同侧大脑半球。在实验II和III中，采用脑电监测或~(14)C-脱氧葡萄糖放射自显影研究癫痫的传播模式。在未切痂的动物中，癫痫的传播有三条途径：1)皮质-皮质通路，2)皮质向基底节和丘脑的病灶，3)皮质-皮质通路，在已切断的动物中，癫痫发作并未加重。然而，放射自显影显示，癫痫发作在两侧大脑半球仍独立存在，本研究建议在临床应用中，应在准确分析癫痫发作类型和发作传播情况后，才能确定癫痫发作的部位。

项目成果

Tanaka T: "Multiple subpial transection in the treatment of neuronal migration disorders : Basic and clinical approaches. In : The Epilepsies : Etiologies and Prevention,"Luders HO,Kotagal P,Academic Press, New York,. 113-120 (1999)

Tanaka T：“多软膜下横断术治疗神经元迁移障碍：基本和临床方法。见：癫痫：病因和预防”，Luders HO，Kotagal P，学术出版社，纽约。

Tanaka T: "Image-guided epilepsy surgery."Neurologica Medico-Chirurgica,. 39. 895-900 (1999)

Tanaka T：“图像引导癫痫手术。”Neurologica Medico-Chirurgica，。

Hashizume K, Tanaka T, Yonemasu Y: "Change in cerebral glucose metabolism during limbic seizures elicited from lateral septal nucleus."Epilpesy research. 30. 167-176 (1998)

Hashizume K、Tanaka T、Yonemasu Y：“侧隔核引起的边缘癫痫发作期间大脑葡萄糖代谢的变化。”癫痫研究。

Hashizume, K, Tanaka T: "Antiepileptic effect of nefiracetam on kainic acid-induced limbic seizure in rats. Epilepsy"Research. 39(3). 221-228 (2000)

Hashizume，K，Tanaka T：“奈非拉西坦对红藻氨酸诱导的大鼠边缘癫痫发作的抗癫痫作用。癫痫”研究。

Tanaka T: "Multiple subpial transection versus callosal section in the treatment of experimentally induced cortical focal seizures"In Epilepsy Surgery, 2^<nd> Edition, (Eds) H O Luders and Y Comair, Bermedica Production, Maryland. 801-806 (2000)

Tanaka T：“多软膜下横断术与胼胝体切片治疗实验诱发的皮质局灶性癫痫”，《癫痫外科》，第 2 版，（编辑）H O Luders 和 Y Comair，Bermedica Production，马里兰州。