Specific loss of chondromodulin-I gene expression in chondrosarcoma and the suppression of tumor angiogenesis and growth by its recombinant protein in vivo

软骨肉瘤中软骨调节素-I基因表达的特异性丧失及其体内重组蛋白对肿瘤血管生成和生长的抑制

• 批准号: 10671350
• 负责人: ENDO Naoto
• 金额: $ 1.66万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671350/
• 关键词: Chondromodulin-I; Angiogenesis inhibitor; Tumor suppression; Tumor angiogenesis; 軟骨肉腫; in situ hybricization; 抗腫瘍作用

Chondromodulin-I (ChM-I) was previously identified as an angiogenesis inhibitor in cartilage. In this study, we showed that the level of ChM-I transcripts was substantially reduced to 100 or even less in the lower-grade chondrosarcomas, in articular cartilage or other benign cartilaginous tumors. We implanted human chondrosarcoma OUMS-27 cells into the back of nude mice that reproducibly produced tumors with cartilaginous matrix. Tumor-induced angiogenesis was evident when the tumors were excised 30 days after implantation. However, the local administration of recombinant human ChM-I almost completely blocked vascular invasion and tumor growth in vivo. Moreover, ChM-I also inhibited the growth of HT-29 colon adenocarcinoma in vivo, implying its therapeutic potential for solid tumors.

软骨调节素-I（ChM-I）以前被鉴定为软骨中的血管生成抑制剂。在这项研究中，我们发现ChM-I转录水平在低级别软骨肉瘤、关节软骨或其他良性软骨肿瘤中显著降低至100甚至更低。我们将人软骨肉瘤OUMS-27细胞植入裸鼠背部，可重复产生具有软骨基质的肿瘤。肿瘤诱导的血管生成是明显的，当肿瘤被切除后30天植入。然而，局部施用重组人ChM-I几乎完全阻断体内血管侵袭和肿瘤生长。ChM-I对HT-29结肠腺癌细胞的生长也有抑制作用，提示其对实体瘤的治疗潜力。

项目成果

Hayami, Tadashi: "Specific loss of chondromodulin-I gene expression in chondrosarcoma and the suppression of tumor angiogenesis and growth by its recombinant protein in vivo"FEBS Letters.. 458-3. 436-440 (1999)

Hayami, Tadashi：“软骨肉瘤中软骨调节素-I 基因表达的特异性丧失及其体内重组蛋白对肿瘤血管生成和生长的抑制”FEBS Letters.. 458-3。

Hayami,Tadashi: "Specific loss of chondromodulin-I gene expression in chondrosarcoma and the suppression of tumor angiogenesis and growth by its recombinant protein in vivo"FEBS Letters. 458・3. 436-440 (1999)

Hayami, Tadashi：“软骨肉瘤中软骨调节蛋白-I 基因表达的特异性丧失及其体内重组蛋白对肿瘤血管生成和生长的抑制”FEBS Letters 458・3 (1999)。

Hayami, Tadashi: "Specific loss of chondromodulin-I gene expression in chondrosarcoma and the suppression of tumor angiogenesis and growth by its recombinant protein in vivo"FEBS Letters. 458. 436-440 (1999)

Hayami, Tadashi：“软骨肉瘤中软骨调节素-I 基因表达的特异性丧失及其体内重组蛋白对肿瘤血管生成和生长的抑制”FEBS Letters。

Hayami T.,Endo N.,et al.: "Specific Loss of Chondromodulin-I(ChM-I:Cartilage Derived Angioinhibitory Factor),mRNA and the Suppression of the Growth by Recombinant ChM-I in Chondrosarcoma" Transaction of the 45th Annual Meeting of Orthopaedic Research Soci

Hayami T.，Endo N.，et al.：“软骨调节素-I（ChM-I：软骨衍生血管抑制因子）、mRNA 的特异性丢失以及重组 ChM-I 在软骨肉瘤中的生长抑制”第 45 届年度交易