Analysis of the differentiation mechanism of human bone marrow derived osteoprogenitor cells

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Because we could not get enough amounts of human bone marrow-derived cells, rats were used in this project to analyze the differentiation me chanism of bone marrow-derived mesencymal cells.1) Establishment of animal model for osteonecrosis of femoral head. Steroid administration could not induce osteonecrosis in rat femoral heads. Since steroid possibly induces ischemia in femoral heads, we tried t o create new osteonecrosis model using rats. We succeeded to create osteonecrosis by cutting the joint capsule and dislocating the femoral he ad. We are now investigating the histological features of the necrosis model including necrotic area and apoptosis.2) Investigation of the bone and cartilage changes in the distal femur of tail-suspended rats. Steroid treated rats had no remarkable changes in bone and cartilage. Since unloading condition could induce osteopenia and fat degeneration i n bone, we changed the animal model to investigate bone and cartilage in tail-suspended rats whose hind limbs were exposed to unloading condition. Unloading induced calcification of articular cartilage and growth inhibition of epiphyseal plate, those were inhibited by intermittent administration of 1-34 human parathyroid hormone.3) Osteoblasitc and adipocytic differentiation of human bone marrow-derived mesenchymal cells inoculated into nude mice Bone marrow-derived mesenchymal cells were inoculated in nude mice. BMP2 induce bone formation, but not adipogenesis in the inoculated mesenchymal cells. These data were not consistent with the in vitro data in which BMP2 induced both osteogenesis and adipogenesis.