Study on Neurotoxicity of Lidocaine

这项研究的目的是为了澄清机制，根据利多卡因诱导的不可逆块被观察到的前两种类型的机制。由于不可逆的，但不是细胞死亡类型块不应该改变阈值，因为它是根据利多凯恩应用程序中的慢性测量确定的。我们在Lidocaine的电压门的钠通道中被拒绝和严重损坏，但进一步的承认没有获得。在另一方手中，我们认为细胞死亡类型的不可逆转的块会导致膜破坏，并导致膜Lysis。为了研究Lidocaine和其他地方的病理学诱导的膜的可能性，以及Hemolysis的可能性，以及由一些地方的病理学确定的米歇尔的形成，并比较了那些排泄物。微囊形成的潜力被表示为关键微囊融合（CMC），通过染料溶解法进行了衡量。Hemolysis的程度被确定为90% Hemolytic concentration （EC-D290-D2）体外。CMC对所有测试的病理学都进行了测量，除了布比卡因。CMC与局部病理学的价值为1/2-1/5，CMC与局部病理学的关系与残留物相同（六烷基三甲基氨C1，Triton-X100），它意味着局部病理学和残留物在同一标记中影响三细胞膜的作用，即膜膜Lysis。

The purpose of this study is to clarify the mechanism underlying the two types of lidocaine induced irreversible block observed previously in crayfish giant axon. As to the irreversible but not cell death type block was not due to threshold change which was confirmed by measurement of chronaxy in crayfish giant axon before and after lidocaine application. We supposed abrupt and serious damage took place in voltage gated Na channels by lidocaine, but further acknowledgement was not acquired. On the other hand, we considered that the cell death type irreversible block was due to membrane destruction, provably membrane lysis. To examine the provability of membrane lysis induced by lidocaine and other local anesthetics, the ability of hemolysis and the formation of micelle were determined with some local anesthetics and compared to those of detergents. Potency of micelle formation was expressed as critical micelle concentration (CMC) measured by dye solubilization method. Degree of hemolysis was determined as 90% hemolytic concentration (ECィイD290ィエD2) in vitro. CMC was measured with all test anesthetics except for bupivacaine. ECィイD290ィエD2 values were 1/2-1/5 of CMC with local anesthetics and this relation was same with detergents (hexadecyl trimethyl ammonium C1, Triton-X100), which means that the local anesthetics and detergents affects erythrocyte membrane in same manner, that is membrane lysis.