Cytodifferentiation on cancer of urogenital tract organs via cell to cell interaction

(A)胎儿或新生间质通过细胞间的相互作用向前列腺癌细胞分化。将所得到的组织重组材料移植到雄性无菌小鼠的肾被膜下1个月。在本实验中，我们可以观察到由未分化的单层或多层鳞状或立方上皮细胞组成的小导管组成的亲代DT的细胞分化。这种组织重组表现为增大的导管，内衬低柱状上皮细胞。然而，大鼠UGM和DT的组织重组显示扩大的导管内排列着高柱状上皮细胞。这种诱导性差异可能是由雄激素…的数量引起的本研究证实间充质细胞通过雄激素受体产生细胞分化因子。人膀胱癌T-24细胞株(T-24)经胎鼠尿生殖窦间充质细胞(大鼠UGM)与大鼠UGM重组后，通过细胞间相互作用向细胞分化。将所产生的组织重组移植到雄性无菌小鼠的肾被膜下1至2个月。在本实验中，我们观察了未分化移行细胞癌细胞T-24的细胞分化情况。这种组织重组表现为扩张的导管内排列着低柱状上皮细胞。前列腺特异性抗原(PSA)局部表达于高柱状上皮细胞。结果表明，胎儿间充质细胞通过上皮-间质相互作用诱导人膀胱癌细胞分化。较少

a) Cytodifferentiation of prostatic cancer cells (Dunning prostatic adenocarcinoma) via cell to cell interaction by various fetal or neonatal mesenchymeThe neonatal rat mesenchymes (uterine and vagina) or fetal rat mesenchymes (esophagus, stomach, small intestine), or urogenital sinus mesenchymes (UGM) of fetal testicular feminization mouse were recombined with R3327 Dunning prostatic adenocarcinoma (DT). The resultant tissue recombinations were grafted under the renal capsule of male athymic mouse for 1 month. In this examination, we could observed the cytodifferentiation of the parental DT composed of small ducts lined by undifferentiated simple or multilayered squamous or cuboidal epithelial cells. Such tissue recombinations showed the enlarged ducts which were lined by low columnar epithelial cells. However, tissue recombination of rat-UGM and DT exhibited the enlarged ducts lined by tall columnar epithelial cells. This inductive difference might be caused by the number of androgen … More receptor in mesenchymal cells.From this result, we could confirm that the mesenchyme produces some cytodifferentiated factors via androgen receptor.Cytodifferentiation of human bladder cancer (T-24) via cell to cell interaction by fetal rat urogenital sinus mesenchyme (rat UGM)Human bladder cancer cells line (T-24) was recombined with rat-UGM. The resultant tissue recombination was grafted under the renal capsule of male athymic mouse for between 1and 2 months. In this examination, we could observed the cytodifferentiation of the T-24 of undifferentiated transitional cell carcinoma cells. Such tissue recombination showed the enlarged ducts which were lined by low columnar epithelial cells. Prostatic specific antigen (PSA) locally expressed on the tall columnar epithelial cells. The epithelial labeling urogenital sinus epithelium combined with rat-UGM.From this result, we could confirm that fetal mesenchyme induce the cytodifferentiation of human bladder cancer cells via epithelial-stromal interaction. Less