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The effect of prostaglandin D synthetase (PGDS) on fertilization, ovum transport and implantation.

前列腺素 D 合成酶 (PGDS) 对受精、卵子运输和着床的影响。

基本信息

批准号：
10671536
负责人：
TOKUGAWA Yosihiro
金额：
$ 2.05万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

In order to elucidate the effect of prostaglandin D synthetase (PGDS) on fertilization, ovum transport and implantation in female reproductive tract, we examine the temporal and spatial expression pattern of PGDS. Messenger RNA of PGDS was detectable in cycling female endometrium and oviduct by Northern blotting. Immunolocalization of PGDS was observed in both of endometrial epithelium in uterus and luminal epithelium of oviduct. No signal was observed in myometrium. As PGDS protein is expressed in the epithelium where fertilization and implantation take place, another question should be whether the expression of this molecule is regulated under sex steroid hormones, especially oestrogen. When we examined immunolocalization of PGDS in the female genital tract treated with GnRH agonist, suppressed intrinsic oestrogen, the signal observed in cycling women had disappeared. This result indicated that the expression of PGDS within the female genital tract is under the control of oestrogen. We further examined the serum level of PGDS, measured by ELISA, in the cycling women. The serum concentration of PGDS was higher in luteal phase than in follicular phase (0.540+-0.081 vs. 0.514+-0.069). Although the difference was statistically significant, the physiological meaning should further be elucidated. In conclusion, we found the PGDS expression in cycling female genital tract epithelium, which is under control of oestrogen. Direct effect of this molecule on fertilization, ovum transfer and implantation should further be examined.

为了阐明前列腺素D合成酶（PGDS）在女性生殖道受精、卵子运输和着床过程中的作用，我们研究了PGDS的时空表达模式。北方印迹法检测到PGDS在周期性子宫内膜和输卵管中的mRNA表达。PGDS在子宫内膜上皮和输卵管腔上皮均有表达。在子宫肌层中未观察到信号。由于PGDS蛋白在受精和着床发生的上皮中表达，另一个问题应该是该分子的表达是否受性类固醇激素，特别是雌激素的调节。当我们检测用GnRH激动剂（抑制内源性雌激素）治疗的女性生殖道中PGDS的免疫定位时，在骑自行车的女性中观察到的信号消失了。提示PGDS在雌性生殖道内的表达受雌激素的调控。我们进一步检查了骑自行车的妇女的血清PGDS水平，用ELISA法测定。黄体期血清PGDS浓度高于卵泡期（0.540+-0.081vs. 0. 514 +-0.069）。虽然差异具有统计学意义，但其生理意义有待进一步阐明。总之，我们发现PGDS在周期性女性生殖道上皮中表达，这是在雌激素的控制下。这种分子对受精、卵子移植和着床的直接影响还有待进一步研究。