Corneal endothelial cell transplantation : Cellular physiological research with expression control of genes transferred into cultivated cell

角膜内皮细胞移植：通过转移到培养细胞中的基因的表达控制进行细胞生理学研究

• 批准号: 10671633
• 负责人: OSHIKA Tetsuro
• 金额: $ 0.77万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671633/
• 关键词: Corneal endothelial transplantation; endothelial cell; proliferation activity; cell culture; cell cycle; 再構築角膜

We conducted the current experimental study to examine the effect of donor age on the morphological variation of cultured human corneal endothelial cells. The human corneal endothelial cells were obtained from the remaining corneoscleral rims of seven human corneas used for penetrating keratoplasty. The donor age ranged from 2 to 75years. Primary cultures were established from explains of the endothelial cell layer, including the Descemet's membrane, and propagated on culture dishes coated with bovine corneal endothelial extracellular matrix. At the fourth passage, average cell area and frequency distribution of cell area in the confluent monolayer were calculated, and the effect of donor age on cell area was analyzed. The percentage of human corneal endothelial cells with cell area over 2000 mmィイD12ィエD1 significantly increased with donor age (r=.935, p=.0007). Furthermore, the average cell area significantly correlated with donor age (r=.920, p=.0015). Cultured human corneal endothelial cells established from older donor tissue display greater average cell area and heterogeneity. The use of human corneal endothelial cells from younger donors is preferable to maximize the benefits of human corneal endothelial cell transplantation.

我们进行了目前的实验研究，以检查供体年龄对培养的人角膜内皮细胞的形态变化的影响。人角膜内皮细胞从用于穿透性角膜移植术的七个人角膜的剩余角巩膜缘获得。供体年龄2 ~ 75岁。从内皮细胞层（包括后弹力膜）的解释建立原代培养物，并在涂有牛角膜内皮细胞外基质的培养皿上繁殖。在第4代时，计算细胞平均面积和细胞面积的频率分布，并分析供体年龄对细胞面积的影响。细胞面积超过2000 mm × 12 × 1此外，平均细胞面积与供体年龄显著相关（r= 0.920，p= 0.0015）。从较老供体组织建立的培养的人角膜内皮细胞显示更大的平均细胞面积和异质性。使用来自年轻供体的人角膜内皮细胞是优选的，以最大化人角膜内皮细胞移植的益处。

项目成果

Miyata K,Drake J, Osakabe Y, Hosokawa Y, Hwang D, Soya K, Oshika T, Amano S: "Effect of donor age on morphological variation of cultured human corneal endothelial cells"Cornea. 19(in press). (2000)

Miyata K、Drake J、Osakabe Y、Hosokawa Y、Hwang D、Soya K、Oshika T、Amano S：“供体年龄对培养的人角膜内皮细胞形态变化的影响”角膜。

Miyata K, Drake J, Osakabe Y, Hosokawa Y, Hwang D, Soya K, Oshika T, Amano S: "Effect of donor age on morphological variation of cultured human corneal endothelial cells"Cornea. 19 (in press). (2000)

Miyata K、Drake J、Osakabe Y、Hosokawa Y、Hwang D、Soya K、Oshika T、Amano S：“供者年龄对培养的人角膜内皮细胞形态变化的影响”角膜。

Miyata K, Drake J, Osakabe Y, Hosokawa Y, Hwang D, Soya K, Oshika T, Amano S: "Effect of donor age on morphological variation of cultured human corneal endothelial cells"Cornea. 19(in press). (2000)

Miyata K、Drake J、Osakabe Y、Hosokawa Y、Hwang D、Soya K、Oshika T、Amano S：“供者年龄对培养的人角膜内皮细胞形态变化的影响”角膜。