Basic and clinical studies of traditional Sino-Japanese(Kampo)medicines on ocular imflammation

中日传统药物治疗眼部炎症的基础与临床研究

• 批准号: 10671636
• 负责人: HAYASAKA Seiji
• 金额: $ 1.73万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671636/
• 关键词: Scutellariae radix; cataract; orengedoku-to; PGE2; LPS; uve : t : s; senkanmeimoku-to; 漢方薬; 前房内フレア値; 白内障術後; 漢方薬(和漢薬); 黄連解毒湯(オウレンゲドクトウ); リポポリサッカライド(エンドトキシン); 眼内炎症; 前房内蛋白濃度; 柴苓湯(サイレイトウ)

We investigate inhibitory effects of Kampo medicines on experimental aqueous flare elevation in pigmented rabbits, and on aqueous flare elevation after cataract surgery in humans. Aqueous flare elevation was induced either by application of prostaglandin E2(PGE2), 25μg/ml, to the cornea, or an intravenous injection of lipopolysaccharides(LPS), 0.5μg/kg, in pigmented rabbits. For pretreatment, about 150g/day of food containing Orengedoku-to, Senkanmeimoku-to, and Scutellariae radix was given to three groups of animals for 5 days before experimental uveitis was induced. Baicalein, baicalin, and wogonin, 60μg/kg or 600μg/kg, was injected into the ear vein 30 minutes before experimentaluveitis. Aqueous flare elevation induced by LPS wes significantly inhibited by pretreatment with Orengedoku-to, Senkanmeimoku-to, baicalein, baicalin, and wogonin. Aqueous flare elevation induced by PGE2 was significantly inhibited by pretreatment with Orengedoku-to, and Senkanmeimoku-to, but not with Scutellariaeradix, baicalein, baicalin, and wogonin.On clinical study, Kakkon-to and Orengedoku-to had inhibitory effects of aqueous flare elevation after cataract surgery.

我们研究了KAMPO药物对实验性房水闪光升高的抑制作用，以及对人类白内障手术后房水闪光升高的抑制作用。将25μg/ml的前列腺素E_2(PGE_2)或0.5μg/kg的脂多糖(LPS)静脉注射于兔角膜，均可引起房水闪光升高。在诱导实验性葡萄膜炎前，三组动物每日给予含有桔梗、千里光、黄芩的饲料约150g/d，共5天。分别于实验性葡萄膜炎前30分钟耳缘静脉注射60μg/kg或600μg/kg的黄芩素、黄芩苷和汉黄素。预先给予桔梗、千里光、黄芩素、黄芩苷和汉黄素可显著抑制脂多糖引起的房水闪光升高。PGE_2引起的房水闪光升高可被桔梗多糖和千里光碱所抑制，而黄芩、黄芩素、黄芩苷和汉黄藤素则不能抑制其升高。临床研究表明，葛根和桔梗对白内障术后房水闪光升高有抑制作用。

项目成果

矢野宏樹,開 繁義,早坂征次: "Effects of Kakkon-to and Sairei-to on experimental elevation of aqueous flare in pigmented rabbits"Japanese Journal of Ophthalmology. 43. 279-284 (1999)

Hiroki Yano、Shigeyoshi Kai、Seiji Hayasaka：“Kakkon-to 和 Sairei-to 对色素兔房水耀斑实验性升高的影响”《日本眼科杂志》43. 279-284 (1999)。

N.Ikeda, Seiji Hayasaka, Y.Nagaki, Y.Hayasaka, C.Kadoi, M.Matsumoto: "Effects of traditional Sino-Japanese herbal medicines on aqueous flare elevation after small-incision cataract surgery"Journal of Ocular Pharmacology and Therapeutics. (in press).

N.Ikeda、Seiji Hayasaka、Y.Nagaki、Y.Hayasaka、C.Kadoi、M.Matsumoto：“中日传统草药对小切口白内障手术后房水耀斑升高的影响”眼部药理学与治疗学杂志。

N.Ikeda, Seiji Hayasaka, Y.Nagaki, Y.Hayasaka, C.Kadol, M.Matsumoto: "Effects of traditional Sino-Japanese herbal medicines on aqueous flare elevation after small-Incision cataract surgery."Journal of Ocular Pharmacology and Therapeutics. (in press).

N.Ikeda、Seiji Hayasaka、Y.Nagaki、Y.Hayasaka、C.Kadol、M.Matsumoto：“中日传统草药对小切口白内障手术后房水耀斑升高的影响”。眼科药理学与治疗杂志

Y.Nagakl,Seiji Hayasaka,C.Kadol,N.Nakamura,Y.Hayasaka: "Effects of Scutellariae radix and its components (balcalein, baicalin, and wogonin) on the experimental elevation of aqueous flare in plgmented rabbits."Japanese Journal of Ophthalmology,. (In press.

Y.Nagakl、Seiji Hayasaka、C.Kadol、N.Nakamura、Y.Hayasaka：“黄芩及其成分（黄芩素、黄芩苷和汉黄芩素）对有色兔子房水耀斑实验性升高的影响。”日本杂志

Y.Nagaki, Seiji Hayasaka, C. Kadoi M.Matsumoto, N.Nakamura, Y.Hayasaka: "Effects of orengedoku-to and senkanmeimoku-to, traditional herbal medicines, on experimental elevation of aqueous flare in pigmented rabbits"American Journal of Chinese Medicine. (in

Y.Nagaki、Seiji Hayasaka、C. Kadoi M.Matsumoto、N.Nakamura、Y.Hayasaka：“传统草药 Orengedoku-to 和 senkanmeimoku-to 对色素兔房水耀斑实验性升高的影响”美国杂志