Basic and clinical studies of traditional Sino-Japanese(Kampo)medicines on ocular imflammation

中日传统药物治疗眼部炎症的基础与临床研究

• 批准号: 10671636
• 负责人: HAYASAKA Seiji
• 金额: $ 1.73万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671636/
• 关键词: Scutellariae radix; cataract; orengedoku-to; PGE2; LPS; uve : t : s; senkanmeimoku-to; 漢方薬; 前房内フレア値; 白内障術後; 漢方薬(和漢薬); 黄連解毒湯(オウレンゲドクトウ); リポポリサッカライド(エンドトキシン); 眼内炎症; 前房内蛋白濃度; 柴苓湯(サイレイトウ)

We investigate inhibitory effects of Kampo medicines on experimental aqueous flare elevation in pigmented rabbits, and on aqueous flare elevation after cataract surgery in humans. Aqueous flare elevation was induced either by application of prostaglandin E2(PGE2), 25μg/ml, to the cornea, or an intravenous injection of lipopolysaccharides(LPS), 0.5μg/kg, in pigmented rabbits. For pretreatment, about 150g/day of food containing Orengedoku-to, Senkanmeimoku-to, and Scutellariae radix was given to three groups of animals for 5 days before experimental uveitis was induced. Baicalein, baicalin, and wogonin, 60μg/kg or 600μg/kg, was injected into the ear vein 30 minutes before experimentaluveitis. Aqueous flare elevation induced by LPS wes significantly inhibited by pretreatment with Orengedoku-to, Senkanmeimoku-to, baicalein, baicalin, and wogonin. Aqueous flare elevation induced by PGE2 was significantly inhibited by pretreatment with Orengedoku-to, and Senkanmeimoku-to, but not with Scutellariaeradix, baicalein, baicalin, and wogonin.On clinical study, Kakkon-to and Orengedoku-to had inhibitory effects of aqueous flare elevation after cataract surgery.

我们研究了汉方药物对有色兔实验性房性闪光升高和人类白内障手术后房性闪光升高的抑制作用。在有色家兔角膜上应用25μg/ml前列腺素E_2（PGE_2）或静脉注射0.5μg/kg脂多糖（LPS），诱发房水闪光升高。作为预处理，在诱导实验性葡萄膜炎之前，向三组动物给予约150 g/天的含有Orengedoku-to、Senkanmeimoku-to和黄芪的食物，持续5天。在实验性输卵管炎前30分钟耳静脉注射黄芩素、黄芩苷和汉黄芩素60μg/kg或600μg/kg。用Orengedoku-to、Senkanmeimoku-to、黄芩素、黄芩苷和汉黄芩素预处理可显著抑制LPS诱导的房水闪光升高。Orengedoku-to和Senkanmeimoku-to预处理可显著抑制PGE 2诱导的房水闪光升高，而黄芩素、黄芩苷和汉黄芩素则无此作用。

项目成果

矢野宏樹,開 繁義,早坂征次: "Effects of Kakkon-to and Sairei-to on experimental elevation of aqueous flare in pigmented rabbits"Japanese Journal of Ophthalmology. 43. 279-284 (1999)

Hiroki Yano、Shigeyoshi Kai、Seiji Hayasaka：“Kakkon-to 和 Sairei-to 对色素兔房水耀斑实验性升高的影响”《日本眼科杂志》43. 279-284 (1999)。

N.Ikeda, Seiji Hayasaka, Y.Nagaki, Y.Hayasaka, C.Kadoi, M.Matsumoto: "Effects of traditional Sino-Japanese herbal medicines on aqueous flare elevation after small-incision cataract surgery"Journal of Ocular Pharmacology and Therapeutics. (in press).

N.Ikeda、Seiji Hayasaka、Y.Nagaki、Y.Hayasaka、C.Kadoi、M.Matsumoto：“中日传统草药对小切口白内障手术后房水耀斑升高的影响”眼部药理学与治疗学杂志。

N.Ikeda, Seiji Hayasaka, Y.Nagaki, Y.Hayasaka, C.Kadol, M.Matsumoto: "Effects of traditional Sino-Japanese herbal medicines on aqueous flare elevation after small-Incision cataract surgery."Journal of Ocular Pharmacology and Therapeutics. (in press).

N.Ikeda、Seiji Hayasaka、Y.Nagaki、Y.Hayasaka、C.Kadol、M.Matsumoto：“中日传统草药对小切口白内障手术后房水耀斑升高的影响”。眼科药理学与治疗杂志

Y.Nagakl,Seiji Hayasaka,C.Kadol,N.Nakamura,Y.Hayasaka: "Effects of Scutellariae radix and its components (balcalein, baicalin, and wogonin) on the experimental elevation of aqueous flare in plgmented rabbits."Japanese Journal of Ophthalmology,. (In press.

Y.Nagakl、Seiji Hayasaka、C.Kadol、N.Nakamura、Y.Hayasaka：“黄芩及其成分（黄芩素、黄芩苷和汉黄芩素）对有色兔子房水耀斑实验性升高的影响。”日本杂志

Y.Nagaki, Seiji Hayasaka, C. Kadoi M.Matsumoto, N.Nakamura, Y.Hayasaka: "Effects of orengedoku-to and senkanmeimoku-to, traditional herbal medicines, on experimental elevation of aqueous flare in pigmented rabbits"American Journal of Chinese Medicine. (in

Y.Nagaki、Seiji Hayasaka、C. Kadoi M.Matsumoto、N.Nakamura、Y.Hayasaka：“传统草药 Orengedoku-to 和 senkanmeimoku-to 对色素兔房水耀斑实验性升高的影响”美国杂志