Regulatory mechanism for gene expression during the development of mouse masseter muscle

小鼠咬肌发育过程中基因表达的调控机制

• 批准号: 10671757
• 负责人: YAMANE Akira
• 金额: $ 1.79万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671757/
• 关键词: rat; masseter muscle; acetylcholine receptor; δ subunit; ε subunit; γ subunit; competitive PCR; development; 舌筋; 後肢筋; α、ε、γサブユニット; MyoDファミリー; mdxマウス; 腓腹筋; ヒラメ筋; 横隔膜; myoD; dystrophin関連遺伝子; 衛生細胞; デスミン; 骨格筋クレアチンキナーゼ; トロポニンC; 発生

There are no published studies on synaptogenesis focusing on the elimination of the nicotinic acetylcholine receptor (nAChR) outside the neuromuscular junction and the nAChR subunit switch from the embryonic-type (α_2βγδ subunits) to the adult-type (α_2βεδ subunits). We analyzed the expression level of δ subunit mRNA, which was present through development, to identify the time course of the nAChR elimination, and the expression level of the ε and γ subunit mRNAs to identify the time course of the nAChR subunit switch in the rat masseter muscle. Rats from 0 to 72 days of age were used, kept in the same cages as their mothers by 18 days of age and then fed a pellet diet thereafter. The mRNA amounts were measured by competitive polymerase chain reaction in combination with reverse transcription. The expression profile of these subunit mRNAs in that rat masseter muscle indicated that the nAChR was mainly eliminated between 7 and 28 days of age and that the nAChR mostly switches from the embryonic to adult types between 1 and 21 days of age. Since the rat feeding behavior seems to switch from suckling to biting after 19 days of age and masseter muscle is known to play an important role in the biting movement of jaw, the completion of the nAChR elimination and switch in the masseter muscle may be related to the switch of the feeding behavior during the postnatal development.

目前还没有关于突触发生的研究集中在神经肌肉接头外的烟碱乙酰胆碱受体（nAChR）的消除和nAChR亚基从胚胎型（α_2βγδ亚基）向成年型（α_2βεδ亚基）的转换。我们分析了δ亚基mRNA的表达水平，这是通过发展，以确定的nAChR消除的时间过程中，和ε和γ亚基mRNA的表达水平，以确定nAChR亚基开关在大鼠咬肌的时间过程。使用0至72日龄的大鼠，在18日龄时与其母亲饲养在同一笼中，然后饲喂颗粒饲料。通过竞争性聚合酶链反应结合逆转录来测量mRNA量。这些亚基mRNA在大鼠咬肌中的表达谱表明，nAChR主要在7至28日龄之间被消除，并且nAChR主要在1至21日龄之间从胚胎型转变为成体型。由于19日龄后大鼠的摄食行为似乎从吮吸转变为咀嚼，而咬肌在颌骨的咬合运动中起着重要作用，因此咬肌中nAChR消除和转换的完成可能与出生后发育过程中摄食行为的转换有关。

项目成果

YAMANE,A.,MAYO,M.,SHULER,C. et al.: "Expression of myogenic regulatory factors during the development of mouse tongue striated muscle"Archs oral Biol.. 45・1. 71-78 (2000)

YAMANE, A.、MAYO, M.、SHULER, C.等：“小鼠舌横纹肌发育过程中生肌调节因子的表达”Archsoral Biol.. 71-78 (2000)。

SAEKI,Y.,OHNUKI,Y.,YAMANE,A. et al.: "Fiber types and their functional role in mammalian masticatory muscles In Neurobiology of mastication - From molecular to systems approach (Eds.Nakamura,Y.and Sessle,B.J.)"Elsevier. 10 (1999)

佐伯 Y.、大贯 Y.、山根 A.

YAMANE,A., BRINGAS,P.Jr., MAYO,M.L., AMANO,O, TAKAHASHI,K., VO,H., SHUM,L.and SLAVKIN,H.C.: "Transforming growth factor alpha up-regulates desmin expression during embryonic mouse tongue myogenesis"Dev.Dyn.. 213(1). 71-78 (1998)

YAMANE,A.、BRINGAS,P.Jr.、MAYO,M.L.、AMANO,O、TAKAHASHI,K.、VO,H.、SHUM,L. 和 SLAVKIN,H.C.：“转化生长因子 α 上调结蛋白表达

YAMANE,A., OHNUKI,Y., SAEKI,Y., YANAGISAWA,K.and CHIBA,M.: "Expression of IGFs, IGF receptors myoD family and acetylcholine receptors during the postnatal development of rat skeletal muscle"In Neurobiology of mastication-From molecular to systems approach

YAMANE,A.、OHNUKI,Y.、SAEKI,Y.、YANAGISAWA,K. 和 CHIBA,M.：“大鼠骨骼肌出生后发育过程中 IGF、IGF 受体 myoD 家族和乙酰胆碱受体的表达”《咀嚼神经生物学》

SAEKI,Y., OHNUKI,Y., YAMANE,A.and YANAGISAWA,K.: "Fiber types and their functional role in mammalian masticatory muscles"In Neurobiology of mastication-From molecular to systems approach (Eds.Nakamura, Y.and Sessle, B.J.), Elsevier, Amsterdam. 327-336 (19

SAEKI,Y.、OHNUKI,Y.、YAMANE,A. 和 YANAGISAWA,K.：“纤维类型及其在哺乳动物咀嚼肌中的功能作用”，咀嚼神经生物学 - 从分子到系统方法（Eds.Nakamura，Y. 和