A study on regulatory mechanism for gene expression in tongue and masseter muscles by adenovirus vector

腺病毒载体对舌和咬肌基因表达调控机制的研究

• 批准号: 13671955
• 负责人: YAMANE Akira
• 金额: $ 1.73万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671955/
• 关键词: mouse; masseter muscle; tongue muscle; growth factors; receptors; binding proteins; competitive PCR; organ culture; 萎縮; インスリン様増殖因子; 結合タンパク質; 免疫組織化学; 肥大; 筋線維タイプ; ミオシン重鎖; クレンブテロール; ラット; インスリン様増殖因子結合蛋白質; カルシニューリン; アデノウイルスベクター

It is well known that peptide growth factors play important roles in skeletal myogenesis. It is also known that tongue striated muscles have several unique characteristics not found in other skeletal muscles, such as limb and trunk muscles, implying that roles of growth factors in tongue myogenesis differ from those in the myogenesis of limb and trunk. Thus, we have studied the roles of insulin-like growth factors (IGFs), hepatocyte growth factor (HGF), and transformig growth factor α(TGFα) in mouse tongue myogenesis. IGF-I promotes the differentiation of tongue myoblasts. HGF promotes the migration and proliferation of tongue myogenic cells, but it inhibits the differentiation of tongue myoblasts. TGFα promotes the differentiation of tongue myoblasts. The roles of IGF-I and HGF in tongue myogenesis seem to be almost identical to those in the myogenesis of limb and cultured myogenic cell lines, such as C2 and L6 ; on the other hand, the role of TGFα seems to differ from them. It is still unknown if the difference in the role of TGFα is ascribed or related to the unique characteristics of tongue striated muscles.

肽类生长因子在骨骼肌发生中起重要作用。舌横纹肌具有肢体和躯干肌等其他骨骼肌所不具备的特性，这表明生长因子在舌肌发生中的作用不同于肢体和躯干肌发生中的作用。因此，我们研究了胰岛素样生长因子（IGFs）、肝细胞生长因子（HGF）和转化生长因子α（TGFα）在小鼠舌肌发生中的作用。IGF-I促进舌肌成肌细胞的分化。HGF促进舌肌原细胞的迁移和增殖，但抑制舌肌母细胞的分化。TGFα促进舌肌成肌细胞的分化。IGF-I和HGF在舌肌发生中的作用似乎与肢体和培养的肌源性细胞系（如C2和L 6）的作用几乎相同;另一方面，TGFα的作用似乎与它们不同。目前尚不清楚TGFα作用的差异是否归因于舌横纹肌的独特特征或与舌横纹肌的独特特征有关。

项目成果

Yamane, A., Ohnuki, Y., Saeki, Y.: "Developmental changes of nicotinic acetylcholine receptor in mouse tongue striated muscle"J.dent.Res.. 80(9). 1840-1844 (2001)

Yamane, A.、Ohnuki, Y.、Saeki, Y.：“小鼠舌横纹肌烟碱乙酰胆碱受体的发育变化”J.dent.Res.. 80(9)。

山根 明, 漆山登喜, 阿久津覚誠, 大井田新一郎: "マウス胎仔の舌を用いた器官培養法"鶴見歯学28(3):315-320,2002. 28・3. 315-320 (2002)

Akira Yamane、Toki Urushiyama、Makoto Akutsu、Shinichiro Oida：“使用小鼠胚胎舌头的器官培养方法” Tsurumi Dentistry 28(3)：315-320，2002. 28・3.315-320 (2002)

URUSHIYAMA, T., AKUTSU, S., MIYAZAKI, J.-L.et al.: "Change from a hard to soft diet alters the expression of insulin-like growth factors, their receptors, and binding proteins in association with atrophy in adult mouse masseter muscle"Cell Tissue Res.. 31

URUSHIYAMA, T., AKUTSU, S., MIYAZAKI, J.-L.等人：“从硬饮食到软饮食的改变改变了胰岛素样生长因子、其受体和与萎缩相关的结合蛋白的表达。

Diekwisch, T.G.H., Bermanb, B.J., Anderton, X., Gurinsky, B., Ortega, A.J., Satchell, P, WIlliams, M., Arumugham, C., Luan, X., Mcintosh, J.E., Yamane, A., Carlson, D.S., Sire, J.-Y., Simmer, J., Shuler, C.F.: "Membranes, minerals, and proteins of develop

Diekwisch, T.G.H.、Bermanb, B.J.、Anderton, X.、Gurinsky, B.、Ortega, A.J.、Satchell, P、WIlliams, M.、Arumugham, C.、Luan, X.、Mcintosh, J.E.、Yamane, A.,

Saito, T., Akutsu, S., Urushiyama, T., Ishibashi, K., Nakagawa, Y., Shuler C.F., Yamane, A.: "Changes in the mRNA expressions of insulin-like growth factors, their receptors and binding proteins during the postnatal development of rat masseter muscle"Zool

Saito, T.、Akutsu, S.、Urushiyama, T.、Ishibashi, K.、Nakakawa, Y.、Shuler C.F.、Yamane, A.：“胰岛素样生长因子及其受体和结合的 mRNA 表达的变化